BBa_J64800
1
BBa_J64800
RHLR/RHLI Inducible & LasR/LasI repressible Promoter
2007-03-24T12:00:00Z
2015-05-08T01:08:17Z
Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLi binding.
false
false
_98_
0
1431
98
Not in stock
false
There is a 17bp spacer between the -35 and -10 regions and the binding site for RHLR/RHLI is 20bp long. I was able to fit the whole binding site in the spacer region because the last three base pairs of the -35 region and the first three base pairs of the RHLR/RHLI binding site are the same.
false
laura lavery
annotation1921061
1
LasR/LasI
range1921061
1
23
40
annotation1921063
1
-10
range1921063
1
42
47
annotation1921062
1
-35
range1921062
1
19
24
annotation1921060
1
RHLR/RHLI
range1921060
1
1
23
BBa_J64800_sequence
1
tcctgtgaaatctggcagttacctgccagttctggcaggtctaaaaagtgttc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z