LOCUS       BBa_J69021              2629 bp    DNA     linear       17-May-2024
DEFINITION  Lux Auto-inducible Device 2
ACCESSION   BBa_J69021
VERSION     BBa_J69021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=BBa_E0030
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
     terminator      812..940
                     /label=BBa_B0015
     misc_feature    1..940
                     /label=BBa_J69524
     stem_loop       949..1077
                     /label=BBa_B0015
     misc_feature    1079..1084
                     /label=SphI restriction site
     CDS             1086..1841
                     /label=BBa_C0062 (luxR), codons optimised for E. coli
     RBS             1848..1859
                     /label=BBa_B0034
     misc_feature    1861..1866
                     /label=BamHI restriction site
     promoter        1868..1922
                     /label=BBa_R0011 (lac, lambda cI hybrid)
     misc_feature    1924..1929
                     /label=BspHI restriction site
     promoter        1931..1985
                     /label=BBa_R0062 (lux pR)
     misc_feature    1987..1992
                     /label=SalI restriction site
     RBS             1994..2005
                     /label=BBa_B0034
     CDS             2012..2629
                     /label=BBa_C0061 (luxI), codons optimised for E. coli
     misc_feature    949..2629
                     /label=BBa_J28031
     misc_feature    949..2629
                     /label=BBa_J28031
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc
      301 gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      541 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagta taaacgcaga
      961 aaggcccacc cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggtgc
     1081 atgcgttatt agtttttgaa atacgggcaa tcgatcgcac cggtcaggat agctttgctg
     1141 atagattgac aacgattcgt ggtgttcagt ttcatctgcg cgttggtcag atggaaagtt
     1201 acggtgcgtt cagagcaacc cagaattttg gagatgtccc aagaggactt accttcgcac
     1261 gcccatgcca ggcattcttt ctcgcgtttg gtcaggtcgt tgttgctttt gttattggca
     1321 atgttaatct tgcgatagtt atcaaccagg gacggaacga tcagaggaat gttcatgcac
     1381 gcgtgcagga acagagaatc gatgtagttg tctttttcgg agtgcgcaaa agacagcata
     1441 ccgaagccgt tgttcgcggt atgaatcggg aagctgaaac cggtgatcag accgctggtt
     1501 tttgcttctt tgataacgtt tggggacttt ttgttaaccg cgttgttctc aaagatattc
     1561 cagttaatcg gggaatggtt agagttagag taatctacga tcggatcgta cttgatcagg
     1621 ttggcgtcat catagtactg acgccatttt tttgggtagt tatccagaat agaaatatcg
     1681 gattttacca tgctgtgcgg ataaatgatg gccagcagat aatattcaca gtgaaccatt
     1741 ttggtcatat cagacaggca ttggttaata tcgttgttag aacgacacgc tttgattttg
     1801 ttaatgatac ggtaggtgtc gtccgcgttg atgtttttca tctagtattt ctcctctttt
     1861 ggatccgtgt gctcagtatc ttgttatccg ctcacaatgt caattgttat ccgctcacaa
     1921 ttttcatgag acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga
     1981 ataaatgtcg acgaaagagg agaaatacta gatgaccatt atgatcaaga aatctgactt
     2041 cctggccatc ccgtccgaag aatacaaagg catcctgtct ctgcgctacc aggttttcaa
     2101 acagcgtctg gaatgggacc tggtggttga aaacaacctg gaatctgacg agtatgataa
     2161 ttccaacgca gaatacatct atgcgtgtga tgatactgaa aacgtttctg gttgttggcg
     2221 tctgctgcct accacgggcg attatatgct gaagtccgta tttccggagc tgctgggtca
     2281 gcagtccgcg ccgaaagacc cgaacattgt agaactgtct cgcttcgcgg ttggcaagaa
     2341 cagctctaaa atcaacaact ccgcaagcga aattaccatg aaactgttcg aagctattta
     2401 taaacacgcg gtttcccagg gtatcaccga atacgtgacc gtcactagca ccgctatcga
     2461 gcgcttcctg aaacgtatca aggttccgtg ccaccgtatc ggtgacaaag aaatccacgt
     2521 actgggtgat accaagagcg tcgtgctgtc catgccgatt aacgagcagt ttaagaaagc
     2581 tgtgctgaac gccgctaacg acgaaaacta cgcgctggtg gcgtaataa
//