LOCUS       BBa_J69582               912 bp    DNA     linear       11-May-2024
DEFINITION  mCherry Reporter
ACCESSION   BBa_J69582
VERSION     BBa_J69582.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23151
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..772
                     /label=mCherry
     misc_feature    413..413
                     /label=C->T (removing PstI site)
     misc_feature    62..775
                     /label=BBa_J06504
     stem_loop       795..838
                     /label=stem_loop
     misc_feature    784..863
                     /label=BBa_B0010
     terminator      784..863
                     /label=BBa_B0010
     stem_loop       879..898
                     /label=T7 TE
     misc_feature    905..905
                     /label=stop
     polyA_site      899..912
                     /label=polya
     misc_feature    872..912
                     /label=BBa_B0012
     terminator      872..912
                     /label=BBa_B0012
     terminator      784..912
                     /label=BBa_B0015
ORIGIN
        1 ttgatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
      121 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      181 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
      241 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
      301 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
      361 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccttgcagga
      421 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
      481 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
      541 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
      601 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
      661 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
      721 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aataatacta
      781 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      841 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      901 tctgcgttta ta
//