LOCUS       BBa_J695910             1852 bp    DNA     linear       12-May-2024
DEFINITION  Ratiometric Test  9102
ACCESSION   BBa_J695910
VERSION     BBa_J695910.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..781
                     /label=GFP protein
     CDS             62..781
                     /label=BBa_E0040
     stem_loop       801..844
                     /label=stem_loop
     misc_feature    790..869
                     /label=BBa_B0010
     terminator      790..869
                     /label=BBa_B0010
     stem_loop       885..904
                     /label=T7 TE
     misc_feature    911..911
                     /label=stop
     polyA_site      905..918
                     /label=polya
     misc_feature    878..918
                     /label=BBa_B0012
     terminator      878..918
                     /label=BBa_B0012
     promoter        964..969
                     /label=-10
     promoter        941..946
                     /label=-35
     misc_feature    951..967
                     /label=OR1
     misc_feature    927..943
                     /label=OR2
     misc_feature    927..975
                     /label=BBa_R0051
     promoter        927..975
                     /label=BBa_R0051
     misc_feature    988..991
                     /label=conserved
     RBS             984..995
                     /label=BBa_B0034
     CDS             1002..1712
                     /label=mCherry
     misc_feature    1353..1353
                     /label=C->T (removing PstI site)
     misc_feature    1002..1715
                     /label=BBa_J06504
     stem_loop       1735..1778
                     /label=stem_loop
     misc_feature    1724..1803
                     /label=BBa_B0010
     terminator      1724..1803
                     /label=BBa_B0010
     stem_loop       1819..1838
                     /label=T7 TE
     misc_feature    1845..1845
                     /label=stop
     polyA_site      1839..1852
                     /label=polya
     misc_feature    1812..1852
                     /label=BBa_B0012
     terminator      1812..1852
                     /label=BBa_B0012
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttatata ctagagtaac accgtgcgtg ttgactattt tacctctggc
      961 ggtgataatg gttgctacta gagaaagagg agaaatacta gatggtgagc aagggcgagg
     1021 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     1081 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1141 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     1201 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1261 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1321 gcggcgtggt gaccgtgacc caggactcct ccttgcagga cggcgagttc atctacaagg
     1381 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     1441 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     1501 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     1561 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     1621 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     1681 ccaccggcgg catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa
     1741 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1801 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//