LOCUS       BBa_J69612              1898 bp    DNA     linear       17-May-2024
DEFINITION  CI Inverter2 Test Device
ACCESSION   BBa_J69612
VERSION     BBa_J69612.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..791
                     /label=cI lambda
     misc_feature    792..824
                     /label=LVA
     misc_feature    831..855
                     /label=Help:Barcodes
     CDS             81..830
                     /label=BBa_C0051
     stem_loop       871..890
                     /label=T7 TE
     misc_feature    897..897
                     /label=stop
     polyA_site      891..904
                     /label=polya
     misc_feature    864..904
                     /label=BBa_B0012
     terminator      864..904
                     /label=BBa_B0012
     stem_loop       925..947
                     /label=stem_loop
     misc_feature    913..958
                     /label=BBa_B0011
     terminator      913..958
                     /label=BBa_B0011
     misc_feature    63..958
                     /label=BBa_P0455
     misc_feature    1..958
                     /label=BBa_J69602
     promoter        1004..1009
                     /label=-10
     promoter        981..986
                     /label=-35
     misc_feature    991..1007
                     /label=OR1
     misc_feature    967..983
                     /label=OR2
     misc_feature    967..1015
                     /label=BBa_R0051
     promoter        967..1015
                     /label=BBa_R0051
     misc_feature    1028..1031
                     /label=conserved
     RBS             1024..1035
                     /label=BBa_B0034
     CDS             1042..1761
                     /label=GFP protein
     CDS             1042..1761
                     /label=BBa_E0040
     stem_loop       1781..1824
                     /label=stem_loop
     misc_feature    1770..1849
                     /label=BBa_B0010
     terminator      1770..1849
                     /label=BBa_B0010
     stem_loop       1865..1884
                     /label=T7 TE
     misc_feature    1891..1891
                     /label=stop
     polyA_site      1885..1898
                     /label=polya
     misc_feature    1858..1898
                     /label=BBa_B0012
     terminator      1858..1898
                     /label=BBa_B0012
     misc_feature    967..1898
                     /label=BBa_J69501
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg
      121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc
      181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg
      241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg
      301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta
      361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga
      421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa
      481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac
      541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg
      601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga
      661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga
      721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag
      781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt
      841 gctagtgtag atcgctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      901 tatatactag agagagaata taaaaagcca gattattaat ccggcttttt tattatttta
      961 ctagagtaac accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgctacta
     1021 gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
     1081 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     1141 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     1201 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
     1261 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     1321 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     1381 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     1441 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     1501 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     1561 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     1621 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1681 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1741 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1801 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1861 cactggctca ccttcgggtg ggcctttctg cgtttata
//