LOCUS       BBa_J69702              1852 bp    DNA     linear       13-May-2024
DEFINITION  R0051:mCherry - Ratiometric (GFP)
ACCESSION   BBa_J69702
VERSION     BBa_J69702.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..786
                     /label=mCherry
     misc_feature    427..427
                     /label=C->T (removing PstI site)
     misc_feature    76..789
                     /label=BBa_J06504
     stem_loop       809..852
                     /label=stem_loop
     misc_feature    798..877
                     /label=BBa_B0010
     terminator      798..877
                     /label=BBa_B0010
     stem_loop       893..912
                     /label=T7 TE
     misc_feature    919..919
                     /label=stop
     polyA_site      913..926
                     /label=polya
     misc_feature    886..926
                     /label=BBa_B0012
     terminator      886..926
                     /label=BBa_B0012
     promoter        935..969
                     /label=BBa_J23101
     misc_feature    982..985
                     /label=conserved
     RBS             978..989
                     /label=BBa_B0034
     CDS             996..1715
                     /label=GFP protein
     CDS             996..1715
                     /label=BBa_E0040
     stem_loop       1735..1778
                     /label=stem_loop
     misc_feature    1724..1803
                     /label=BBa_B0010
     terminator      1724..1803
                     /label=BBa_B0010
     stem_loop       1819..1838
                     /label=T7 TE
     misc_feature    1845..1845
                     /label=stop
     polyA_site      1839..1852
                     /label=polya
     misc_feature    1812..1852
                     /label=BBa_B0012
     terminator      1812..1852
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat catcaaggag
      121 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      181 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      241 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      301 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      361 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      421 tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      481 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      541 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      601 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      661 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      721 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      781 aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      901 ttcgggtggg cctttctgcg tttatatact agagtttaca gctagctcag tcctaggtat
      961 tatgctagct actagagaaa gaggagaaat actagatgcg taaaggagaa gaacttttca
     1021 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     1081 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     1141 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
     1201 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     1261 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     1321 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     1381 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     1441 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     1501 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     1561 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     1621 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     1681 ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa
     1741 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1801 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//