BBa_J70040

BBa_J70040 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J70040

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julie Norville

Date created: 2009-01-22 12:00:00

Date modified: 2015-05-08 01:08:20

A BioScaffold Part (Uses AarI, MabI) for Library Vector Preparation (see Part Design page)

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_J70040_sequence (Version 1)

Description

When placed in a BioBrick assembly and cut with the restriction enzyme AarI, this part will direct the restriction enzyme to cut within the BioBrick scar
still in testing, uses AarI (2), MabI(2), XmaI (1) * XmaI does not work in vector for BioScaffold part manipulations
See part design page or BBFRFC15, for details about use

Notes

See BBa_J70034, this is an analogous part except it uses MabI

Source

Designed

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
AarI	1,7	sequence_feature, feature/misc
AarI	38,44	feature/misc, sequence_feature
XmaI	20,25	feature/binding, non_covalent_binding_site
MabI	11,17	sequence_feature, feature/dna
MabI	29,34	sequence_feature, feature/dna

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J70040/1

Member of these Collections

• //dna/bioscaffold
• iGEM Parts Registry