BBa_J70042

BBa_J70042 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J70042

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julie Norville

Date created: 2009-01-22 12:00:00

Date modified: 2015-05-08 01:08:20

6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)

Details

• Types: DnaRegion
• Roles: tag; Tag
• Sequences: BBa_J70042_sequence (Version 1)

Description

This 7 His prefix tag and be used with BBa_J70010 (PpiI) and BBa_J70012 (PsrI) in fusing a 6 his tag to the N-terminus of a protein.
This part is still in testing.
During the BioScaffold part excision, it should be placed in pSB2K3.

Notes

This can be used when creating standard fusions between the 6 his tag and protein parts using BioScaffold parts.

Source

Designed.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start	1,3	feature/start, start_codon
6 his	4,21	CDS, feature/cds
gly-ser	22,27	feature/dna, sequence_feature
N terminal 6 his	1,27	tag, feature/tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J70042/1

Member of these Collections

• iGEM Parts Registry