LOCUS       BBa_J70577              1969 bp    DNA     linear       12-Jun-2024
DEFINITION  C-terminal protein tag addition test circuit
ACCESSION   BBa_J70577
VERSION     BBa_J70577.1  
FEATURES             Location/Qualifiers
     promoter        1..166
                     /label=BsaXI removed, R0010
     RBS             175..186
                     /label=B0034
     CDS             194..912
                     /label=E0040
     misc_feature    1..912
                     /label=BBa_J70570
     misc_feature    921..924
                     /label=BsaXI partial site (only occurs when follows scar)
     misc_feature    1715..1725
                     /label=BsaXI site
     misc_feature    1960..1969
                     /label=BsaXI partial site (occurs with suffix or scar)
     misc_feature    1426..1426
                     /label=mutation from J70568: G506A (DNA), V169I (protein)
     promoter        921..1090
                     /label=R0010(-naturally occurring internal BsaXI site)
     RBS             1098..1110
                     /label=B0034
     CDS             1117..1797
                     /label=E1010
     misc_feature    1798..1822
                     /label=Junk from J04450
     misc_feature    932..934
                     /label=BsaXI potential cut site
     misc_feature    1703..1705
                     /label=BsaXI cut site
     misc_feature    1733..1735
                     /label=BsaXI cut site
     misc_feature    1950..1952
                     /label=BsaXI cut site
     misc_feature    1831..1910
                     /label=B0010
     misc_feature    1919..1959
                     /label=B0012
     misc_feature    921..1969
                     /label=BBa_J70575
ORIGIN
        1 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
       61 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      121 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag
      181 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      241 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      301 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      361 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
      421 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      481 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      541 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      601 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      661 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
      721 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      781 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
      841 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
      901 tacaaataat aatactagag ctccgattca ttaatgcagc tggcacgaca ggtttcccga
      961 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     1021 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     1081 atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     1141 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1201 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1261 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1321 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1381 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtattgt taccgttacc
     1441 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     1501 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     1561 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     1621 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     1681 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     1741 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     1801 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt
     1861 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1921 acactggctc accttcgggt gggcctttct gcgtttatag gaggacagg
//