BBa_J70584 1 BBa_J70584 pyrrolysyl-tRNA synthetase (PylRS)???tRNAPyl (pylT) 2010-05-17T11:00:00Z 2015-05-08T01:08:24Z web, 4.2 Construction of pBK-PylRS The plasmid pBK-PylRS was derived from pBK-JYRS.[7] The gene of Methanosarcina mazei PylRS was PCR amplified from Methanosarcina mazei genomic DNA which was purchased from ATCC. Two restriction sites, Nde1 at the 5??? end and Pst1 at the 3??? end, were introduced in the PCR product which was subsequently digested and cloned into pBK-JYRS to replace JYRS. Methanosarcina mazei Go1, complete genome pylS pyrolysyl-tRNA synthetase [ Methanosarcina mazei Go1 ] false false _41_ 0 1201 41 Not in stock false none false Julie Norville BBa_J70584_sequence 1 atggataaaaaaccactaaacactctgatatctgcaaccgggctctggatgtccaggaccggaacaattcataaaataaaacaccacgaagtctctcgaagcaaaatctatattgaaatggcatgcggagaccaccttgttgtaaacaactccaggagcagcaggactgcaagagcgctcaggcaccacaaatacaggaagacctgcaaacgctgcagggtttcggatgaggatctcaataagttcctcacaaaggcaaacgaagaccagacaagcgtaaaagtcaaggtcgtttctgcccctaccagaacgaaaaaggcaatgccaaaatccgttgcgagagccccgaaacctcttgagaatacagaagcggcacaggctcaaccttctggatctaaattttcacctgcgataccggtttccacccaagagtcagtttctgtcccggcatctgtttcaacatcaatatcaagcatttctacaggagcaactgcatccgcactggtaaaagggaatacgaaccccattacatccatgtctgcccctgttcaggcaagtgcccccgcacttacgaagagccagactgacaggcttgaagtcctgttaaacccaaaagatgagatttccctgaattccggcaagcctttcagggagcttgagtccgaattgctctctcgcagaaaaaaagacctgcagcagatctacgcggaagaaagggagaattatctggggaaactcgagcgtgaaattaccaggttctttgtggacaggggttttctggaaataaaatccccgatcctgatccctcttgagtatatcgaaaggatgggcattgataatgataccgaactttcaaaacagatcttcagggttgacaagaacttctgcctgagacccatgcttgctccaaacctttacaactacctgcgcaagcttgacagggccctgcctgatccaataaaaatttttgaaataggcccatgctacagaaaagagtccgacggcaaagaacacctcgaagagtttaccatgctgaacttctgccagatgggatcgggatgcacacgggaaaatcttgaaagcataattacggacttcctgaaccacctgggaattgatttcaagatcgtaggcgattcctgcatggtctatggggatacccttgatgtaatgcacggagacctggaactttcctctgcagtagtcggacccataccgcttgaccgggaatggggtattgataaaccctggataggggcaggtttcgggctcgaacgccttctaaaggttaaacacgactttaaaaatatcaagagagctgcaaggtccgagtcttactataacgggatttctaccaacctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z