BBa_J70594
1
BBa_J70594
RFC12 TAATAA Tail Domain
2010-06-17T11:00:00Z
2015-05-08T01:08:25Z
Common Knowledge
A RFC12 compatible part that simply codes for two stop codons. This part does not have any degradation tag.
false
true
_41_
0
6384
41
Not in stock
false
Made with synthetic oligos:
5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3'
5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3'
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
false
Joseph Lynch
annotation2071257
1
stop
range2071257
1
1
5
BBa_J70595
1
BBa_J70595
RFC12 Green Fluorescent Protein
2010-06-22T11:00:00Z
2015-05-08T01:08:25Z
BBa_E0040
This is a RFC12 compatible part based off of BBa_E0040.
false
true
_41_
0
6384
41
It's complicated
false
Amplified via colony PCR directly from the BBa_E0040 cell stock.
Forward Primer:
5' GTTTCTT C GAATTC GCGGCGC T ACTAGT CGTAAAGGAGAAGAACTTTTCACTG 3'
Reverse Primer:
3' CACATGGCATGGATGAACTATACAAA 5'
so
5' GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTTGTATAGTTCATCCATGCCATGTG 3'
false
Joseph Lynch
annotation2071481
1
GFP
range2071481
1
1
710
BBa_J70614
1
BBa_J70614
RFC12 His tagged GFP
2010-07-21T11:00:00Z
2015-05-08T01:08:25Z
<partinfo>J70611</partinfo>,<partinfo>J70595</partinfo>,<partinfo>J70594</partinfo>
This is an N-terminal fusion of a His tag onto a GFP internal domain. It conforms with the RFC12 assembly standard and therefore supports fusions.
'''NOTE''':
The assembly scars shown below as of July 22, 2010 are incorrect. The correct assembly scar is GCTAGT. This codes for Ala-Ser.
false
false
_41_
0
6384
41
Not in stock
false
None
false
Joseph Lynch
component2075343
1
BBa_J70594
component2075341
1
BBa_J70595
component2075339
1
BBa_J70609
annotation2075343
1
BBa_J70594
range2075343
1
755
760
annotation2075341
1
BBa_J70595
range2075341
1
36
746
annotation2075339
1
BBa_J70609
range2075339
1
1
27
BBa_J70609
1
BBa_J70609
RFC12 7x His Tag Head Domain
2010-07-15T11:00:00Z
2015-05-08T01:08:25Z
Registry part BBa_T2001.
This is a 7x His tag which is commonly used to tag proteins for purification purposes. It is an RFC12 compatible head domain mean for N-terminal fusions.
false
false
_41_
0
6384
41
Not in stock
false
Constructed using oligos:
AATTC GCGGCCGC T ACTAGT ATGAAACACCATCACCATCACCATCAC GCTAGC A GCGGCCG CTGCA, His-F
G CGGCCGC T GCTAGC GTGATGGTGATGGTGATGGTGTTTCAT ACTAGT A GCGGCCGC G
false
Joseph Lynch
annotation2073951
1
Preferred AAA +2 codon
range2073951
1
4
6
annotation2073952
1
7x His Tag
range2073952
1
7
25
annotation2073950
1
start codon
range2073950
1
1
3
BBa_J70595_sequence
1
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa
BBa_J70614_sequence
1
atgaaacaccatcaccatcaccatcactactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagagtaataa
BBa_J70594_sequence
1
taataa
BBa_J70609_sequence
1
atgaaacaccatcaccatcaccatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z