BBa_J70594 1 BBa_J70594 RFC12 TAATAA Tail Domain 2010-06-17T11:00:00Z 2015-05-08T01:08:25Z Common Knowledge A RFC12 compatible part that simply codes for two stop codons. This part does not have any degradation tag. false true _41_ 0 6384 41 Not in stock false Made with synthetic oligos: 5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3' 5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3' Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase. false Joseph Lynch annotation2071257 1 stop range2071257 1 1 5 BBa_J70595 1 BBa_J70595 RFC12 Green Fluorescent Protein 2010-06-22T11:00:00Z 2015-05-08T01:08:25Z BBa_E0040 This is a RFC12 compatible part based off of BBa_E0040. false true _41_ 0 6384 41 It's complicated false Amplified via colony PCR directly from the BBa_E0040 cell stock. Forward Primer: 5' GTTTCTT C GAATTC GCGGCGC T ACTAGT CGTAAAGGAGAAGAACTTTTCACTG 3' Reverse Primer: 3' CACATGGCATGGATGAACTATACAAA 5' so 5' GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTTGTATAGTTCATCCATGCCATGTG 3' false Joseph Lynch annotation2071481 1 GFP range2071481 1 1 710 BBa_J70614 1 BBa_J70614 RFC12 His tagged GFP 2010-07-21T11:00:00Z 2015-05-08T01:08:25Z <partinfo>J70611</partinfo>,<partinfo>J70595</partinfo>,<partinfo>J70594</partinfo> This is an N-terminal fusion of a His tag onto a GFP internal domain. It conforms with the RFC12 assembly standard and therefore supports fusions. '''NOTE''': The assembly scars shown below as of July 22, 2010 are incorrect. The correct assembly scar is GCTAGT. This codes for Ala-Ser. false false _41_ 0 6384 41 Not in stock false None false Joseph Lynch component2075343 1 BBa_J70594 component2075341 1 BBa_J70595 component2075339 1 BBa_J70609 annotation2075343 1 BBa_J70594 range2075343 1 755 760 annotation2075341 1 BBa_J70595 range2075341 1 36 746 annotation2075339 1 BBa_J70609 range2075339 1 1 27 BBa_J70609 1 BBa_J70609 RFC12 7x His Tag Head Domain 2010-07-15T11:00:00Z 2015-05-08T01:08:25Z Registry part BBa_T2001. This is a 7x His tag which is commonly used to tag proteins for purification purposes. It is an RFC12 compatible head domain mean for N-terminal fusions. false false _41_ 0 6384 41 Not in stock false Constructed using oligos: AATTC GCGGCCGC T ACTAGT ATGAAACACCATCACCATCACCATCAC GCTAGC A GCGGCCG CTGCA, His-F G CGGCCGC T GCTAGC GTGATGGTGATGGTGATGGTGTTTCAT ACTAGT A GCGGCCGC G false Joseph Lynch annotation2073951 1 Preferred AAA +2 codon range2073951 1 4 6 annotation2073952 1 7x His Tag range2073952 1 7 25 annotation2073950 1 start codon range2073950 1 1 3 BBa_J70595_sequence 1 cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa BBa_J70614_sequence 1 atgaaacaccatcaccatcaccatcactactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagagtaataa BBa_J70594_sequence 1 taataa BBa_J70609_sequence 1 atgaaacaccatcaccatcaccatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z