LOCUS BBa_J85108 2732 bp DNA linear 17-May-2024 DEFINITION pLac:RBS:LuxI:pLacIQ:RBS:LuxR:TT:pLux:RBS:RFP ACCESSION BBa_J85108 VERSION BBa_J85108.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..811 /label=LuxI CDS 227..811 /label=BBa_C0161 stem_loop 831..874 /label=stem_loop misc_feature 820..899 /label=BBa_B0010 terminator 820..899 /label=BBa_B0010 stem_loop 915..934 /label=T7 TE misc_feature 941..941 /label=stop polyA_site 935..948 /label=polya misc_feature 908..948 /label=BBa_B0012 terminator 908..948 /label=BBa_B0012 misc_feature 957..993 /label=P(Lac) IQ promoter 959..964 /label=-35 promoter 982..987 /label=-10 promoter 957..993 /label=BBa_I14032 misc_feature 1006..1009 /label=conserved RBS 1002..1013 /label=BBa_B0034 misc_feature 1020..1021 /label=prefix misc_feature 1193..1193 /label=T misc_feature 1511..1511 /label=A CDS 1020..1769 /label=luxR misc_feature 1020..1775 /label=BBa_C0062 misc_feature 1776..1800 /label=Help:Barcodes CDS 1020..1775 /label=BBa_C0062 stem_loop 1820..1863 /label=stem_loop misc_feature 1809..1888 /label=BBa_B0010 terminator 1809..1888 /label=BBa_B0010 stem_loop 1904..1923 /label=T7 TE misc_feature 1930..1930 /label=stop polyA_site 1924..1937 /label=polya misc_feature 1897..1937 /label=BBa_B0012 terminator 1897..1937 /label=BBa_B0012 misc_feature 1002..1937 /label=BBa_I0462 misc_feature 1946..1965 /label=LuxR/HSL promoter 1965..1970 /label=-35 promoter 1987..1992 /label=-10 misc_feature 1998..1998 /label=start misc_feature 1946..2000 /label=BBa_R0062 promoter 1946..2000 /label=BBa_R0062 misc_feature 957..2000 /label=BBa_J09855 misc_feature 2013..2016 /label=conserved RBS 2009..2020 /label=BBa_B0034 CDS 2027..2701 /label=mrfp1 misc_feature 2708..2732 /label=Help:Barcodes CDS 2027..2707 /label=BBa_E1010 misc_feature 2009..2732 /label=BBa_I13502 misc_feature 957..2732 /label=BBa_J10000 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat 241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg 301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc 361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt 421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc 481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt 541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact 601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac 661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga 721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga 781 acagtttaaa aaagcagtct taaattaata atactagagc caggcatcaa ataaaacgaa 841 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 901 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtggt 961 gcaaaacctt tcgcggtatg gcatgatagc gcctactaga gaaagaggag aaatactaga 1021 tgaaaaacat aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa 1081 gcaataatga tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt 1141 atttactcgc gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata 1201 attaccctaa aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta 1261 tagtagatta ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg 1321 ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg 1381 ggtttagttt ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt 1441 cagaaaaaga caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa 1501 ttgttccttc tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca 1561 acgatttaac caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt 1621 gggatatttc aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg 1681 cgcaaatgaa actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag 1741 gagcaattga ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac 1801 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1861 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1921 gcctttctgc gtttatatac tagagacctg taggatcgta caggtttacg caagaaaatg 1981 gtttgttata gtcgaataaa tactagagaa agaggagaaa tactagatgg cttcctccga 2041 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca 2101 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa 2161 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt 2221 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact 2281 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt 2341 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg 2401 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc 2461 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct 2521 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa 2581 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa 2641 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc 2701 ttaataacgc tgatagtgct agtgtagatc gc //