LOCUS       BBa_J85139              1756 bp    DNA     linear       03-Jun-2024
DEFINITION  error correction part 2
ACCESSION   BBa_J85139
VERSION     BBa_J85139.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    69..72
                     /label=conserved
     RBS             63..76
                     /label=BBa_B0031
     CDS             86..793
                     /label=cI lambda
     misc_feature    794..826
                     /label=LVA
     misc_feature    833..857
                     /label=Help:Barcodes
     CDS             83..832
                     /label=BBa_C0051
     stem_loop       877..920
                     /label=stem_loop
     misc_feature    866..945
                     /label=BBa_B0010
     terminator      866..945
                     /label=BBa_B0010
     stem_loop       961..980
                     /label=T7 TE
     misc_feature    987..987
                     /label=stop
     polyA_site      981..994
                     /label=polya
     misc_feature    954..994
                     /label=BBa_B0012
     terminator      954..994
                     /label=BBa_B0012
     misc_feature    63..994
                     /label=BBa_P0151
     misc_feature    1003..1023
                     /label=LuxR-AHL Binding Box
     misc_feature    1044..1049
                     /label=Pseudo-Pribnow Box
     misc_feature    1054..1070
                     /label=Lambda Repressor OR1
     misc_feature    1053..1053
                     /label=Transcription Start Site
     promoter        1003..1070
                     /label=BBa_K415032
     misc_feature    1083..1086
                     /label=conserved
     RBS             1079..1090
                     /label=BBa_B0034
     misc_feature    1097..1756
                     /label=CmR gene
     CDS             1097..1756
                     /label=BBa_J31005
     misc_feature    1079..1756
                     /label=BBa_J85101
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaacctact agatgagcac aaaaaagaaa ccattaacac aagagcagct
      121 tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc
      181 ccaggaatct gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa
      241 tggcatcaat gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag
      301 cgttgaagaa tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag
      361 tatgcagccg tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg
      421 gatgttctca cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac
      481 aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc
      541 accaacaggc tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca
      601 ggctgttgag ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa
      661 gaaactgatc agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat
      721 gatcccatgc aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga
      781 agagacgttt ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata
      841 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      901 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      961 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag gatcgtacag
     1021 gtttacgcaa gaaaatggtt tgttatagtc gaatacctct ggcggtgata tactagagaa
     1081 agaggagaaa tactagatgg agaaaaaaat cactggatat accaccgttg atatatccca
     1141 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     1201 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     1261 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat
     1321 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     1381 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     1441 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     1501 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     1561 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     1621 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     1681 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     1741 gcagggcggg gcgtaa
//