LOCUS       BBa_J85800              2278 bp    DNA     linear       18-May-2024
DEFINITION  LacI-T2A-EBFP2-FF5-miRFF4
ACCESSION   BBa_J85800
VERSION     BBa_J85800.1  
FEATURES             Location/Qualifiers
     CDS             8..1123
                     /label=LacI
     misc_feature    1124..1177
                     /label=T2A
     CDS             1178..1897
                     /label=EBFP2
     misc_feature    1898..1908
                     /label=splice site 1
     stem_loop       1909..2257
                     /label=miRNA FF4
     misc_feature    2258..2278
                     /label=splice site 2
ORIGIN
        1 tcccaccatg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
       61 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
      121 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
      181 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
      241 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
      301 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
      361 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
      421 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
      481 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
      541 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
      601 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
      661 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
      721 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
      781 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
      841 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
      901 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
      961 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     1021 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1081 cgggcagagc agcctgaggc ctcctaagaa gaagaggaag gttgagggcc gcggcagcct
     1141 gctgacctgc ggcgacgtgg aggaaaaccc cggccccatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1261 cagcgtgagg ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgagccacgg
     1381 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcacctacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctagtg aaccgcatcg agctgaaggg
     1561 cgtcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     1621 ccacaacatc tatatcatgg ccgtcaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac gtggaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga cagccactac ctgagcaccc agtccgtgct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcc gcaccgccgc
     1861 cgggatcact ctcggcatgg acgagctgta caagtaagag gtgagtatgt gctcgcttcg
     1921 gcagcacata tactatgttg aatgaggctt cagtacttta cagaatcgtt gcctgcacat
     1981 cttggaaaca cttgctggga ttacttcttc aggttaaccc aacagaaggc tcgagtgctg
     2041 ttgacagtga gcgccgcttg aagtttttaa ttaaatagtg aagccacaga tgtatttaat
     2101 taaagacttc aagcggtgcc tactgcctcg gagaattcaa ggggctactt taggagcaat
     2161 tatcttgttt actaaaactg aataccttgc tatctctttg atacattttt acaaagctga
     2221 attaaaatgg tataaattaa atcacttttt tcaattgttt cctttttttt cctcaggg
//