LOCUS       BBa_J85900              2630 bp    DNA     linear       17-May-2024
DEFINITION  TRE-tight-B1-LacI-T2A-EBFP2-FF5-miRFF4
ACCESSION   BBa_J85900
VERSION     BBa_J85900.1  
FEATURES             Location/Qualifiers
     misc_feature    30..35
                     /label=Bind_Site_1
     misc_feature    46..63
                     /label=tetO reg
     misc_feature    81..96
                     /label=tetO reg
     misc_feature    117..134
                     /label=tetO reg
     misc_feature    153..170
                     /label=tetO reg
     misc_feature    188..205
                     /label=tetO reg
     misc_feature    224..241
                     /label=tetO reg
     promoter        256..313
                     /label=CMV-minimal
     misc_feature    314..315
                     /label=Bind_Site_2
     promoter        1..330
                     /label=BBa_J85700
     misc_feature    332..352
                     /label=attB1
     misc_feature    331..352
                     /label=BBa_J85600
     CDS             360..1475
                     /label=LacI
     misc_feature    1476..1529
                     /label=T2A
     CDS             1530..2249
                     /label=EBFP2
     misc_feature    2250..2260
                     /label=splice site 1
     stem_loop       2261..2609
                     /label=miRNA FF4
     misc_feature    2610..2630
                     /label=splice site 2
     CDS             353..2630
                     /label=BBa_J85800
ORIGIN
        1 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcaaaggg cgaattcgac ccaagtttgt acaaaaaagc agtcccacca
      361 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
      421 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
      481 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
      541 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
      601 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
      661 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
      721 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
      781 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
      841 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
      901 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
      961 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     1021 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     1081 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     1141 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     1201 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     1261 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     1321 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     1381 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcaga
     1441 gcagcctgag gcctcctaag aagaagagga aggttgaggg ccgcggcagc ctgctgacct
     1501 gcggcgacgt ggaggaaaac cccggcccca tggtgagcaa gggcgaggag ctgttcaccg
     1561 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtga
     1621 ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca
     1681 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagccac ggcgtgcagt
     1741 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1801 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcacctac aagacccgcg
     1861 ccgaggtgaa gttcgagggc gacaccctag tgaaccgcat cgagctgaag ggcgtcgact
     1921 tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac agccacaaca
     1981 tctatatcat ggccgtcaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     2041 acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     2101 acggccccgt gctgctgccc gacagccact acctgagcac ccagtccgtg ctgagcaaag
     2161 accccaacga gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc gccgggatca
     2221 ctctcggcat ggacgagctg tacaagtaag aggtgagtat gtgctcgctt cggcagcaca
     2281 tatactatgt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa
     2341 cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagtgc tgttgacagt
     2401 gagcgccgct tgaagttttt aattaaatag tgaagccaca gatgtattta attaaagact
     2461 tcaagcggtg cctactgcct cggagaattc aaggggctac tttaggagca attatcttgt
     2521 ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat
     2581 ggtataaatt aaatcacttt tttcaattgt ttcctttttt ttcctcaggg
//