LOCUS       BBa_J85901              2744 bp    DNA     linear       18-May-2024
DEFINITION  Hef1a-B1-rtTA3-2A-NLS-mKate2
ACCESSION   BBa_J85901
VERSION     BBa_J85901.1  
FEATURES             Location/Qualifiers
     promoter        23..1196
                     /label=pHef1A
     promoter        1..1218
                     /label=BBa_J85701
     misc_feature    1220..1240
                     /label=attB1
     misc_feature    1219..1240
                     /label=BBa_J85600
     CDS             1250..1954
                     /label=rtTA3
     misc_feature    1961..2014
                     /label=2A
     misc_feature    2021..2044
                     /label=NLS
     CDS             2045..2740
                     /label=mKate2
     CDS             1241..2744
                     /label=BBa_J85801
ORIGIN
        1 gctccgaatt cgcccttatt aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg
       61 cccacagtcc ccgagaagtt gtggggaggg gtcggcaatt gaaccggtgc ctagagaagg
      121 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
      181 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
      241 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
      301 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
      361 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
      421 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
      481 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
      541 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
      601 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
      661 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
      721 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
      781 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
      841 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
      901 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
      961 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     1021 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     1081 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     1141 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtggat
     1201 cccaagggcg aattcgaccc aagtttgtac aaaaaagcag gacgccacca tgtctaggct
     1261 ggacaagagc aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg
     1321 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
     1381 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
     1441 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
     1501 gtcataccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
     1561 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
     1621 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
     1681 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
     1741 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
     1801 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
     1861 aagcggcggg ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc
     1921 ccttgacgat tttgaccttg acatgctccc cggggaattg gagggccgcg gcagcctgct
     1981 gacctgcggc gacgtggagg aaaaccccgg ccccatggtg ccaccaaaaa aaaaacgcaa
     2041 agttgtgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     2101 gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     2161 gaccatgaga atcaaggcgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     2221 taccagcttc atgtacggca gcaaaacctt catcaaccac acccagggca tccccgactt
     2281 ctttaagcag tccttccccg agggcttcac atgggagaga gtcaccacat acgaagacgg
     2341 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     2401 caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg
     2461 ctgggaggcc tccaccgaga ccctgtaccc cgctgacggc ggcctggaag gcagagccga
     2521 catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttgaaga ccacatacag
     2581 atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg acagaagact
     2641 ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc
     2701 cagatactgc gacctcccta gcaaactggg gcacagatga tacc
//