LOCUS       BBa_J85902              2197 bp    DNA     linear       17-May-2024
DEFINITION  Hef1a-LacO1Oid-B1-EYFP-FF4x4
ACCESSION   BBa_J85902
VERSION     BBa_J85902.1  
FEATURES             Location/Qualifiers
     promoter        16..1290
                     /label=Hef1a
     misc_feature    216..236
                     /label=lacO1
     misc_feature    287..306
                     /label=lacOid
     promoter        1..1315
                     /label=BBa_J85702
     misc_feature    1317..1337
                     /label=attB1
     misc_feature    1316..1337
                     /label=BBa_J85600
     CDS             1373..2092
                     /label=EYFP
     stem_loop       2104..2125
                     /label=FF4
     stem_loop       2126..2147
                     /label=FF4
     stem_loop       2148..2169
                     /label=FF4
     stem_loop       2170..2191
                     /label=FF4
     CDS             1338..2197
                     /label=BBa_J85802
ORIGIN
        1 gctccgaatt ctaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
       61 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
      121 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      181 accgtatata agtgcagtag tcgccgtgaa gctagaattg tgagcggata acaattccac
      241 agtcgaccct aggttgtgtc gcgagtgttg gatcccagct gacaccaatt gtgagcgctc
      301 acaattgcta gccgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
      361 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
      421 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
      481 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
      541 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
      601 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
      661 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
      721 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
      781 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
      841 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
      901 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
      961 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     1021 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     1081 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     1141 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     1201 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     1261 ggttcaaagt ttttttcttc catttcaggt gaagacttga tccccggaat tcgacccaag
     1321 tttgtacaaa aaagcagttc ttccatttca ggtggatcca ccggtcgcca ccatggtgag
     1381 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1441 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1501 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1561 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     1621 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1681 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1741 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1801 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1861 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1921 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1981 ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2041 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggcgc
     2101 gccccgcttg aagtctttaa ttaaaccgct tgaagtcttt aattaaaccg cttgaagtct
     2161 ttaattaaac cgcttgaagt ctttaattaa agctagt
//