LOCUS BBa_J85950 7661 bp DNA linear 17-May-2024 DEFINITION LacI inverter regulated by TRE ACCESSION BBa_J85950 VERSION BBa_J85950.1 FEATURES Location/Qualifiers misc_feature 30..35 /label=Bind_Site_1 misc_feature 46..63 /label=tetO reg misc_feature 81..96 /label=tetO reg misc_feature 117..134 /label=tetO reg misc_feature 153..170 /label=tetO reg misc_feature 188..205 /label=tetO reg misc_feature 224..241 /label=tetO reg promoter 256..313 /label=CMV-minimal misc_feature 314..315 /label=Bind_Site_2 promoter 1..330 /label=BBa_J85700 misc_feature 332..352 /label=attB1 misc_feature 331..352 /label=BBa_J85600 CDS 360..1475 /label=LacI misc_feature 1476..1529 /label=T2A CDS 1530..2249 /label=EBFP2 misc_feature 2250..2260 /label=splice site 1 stem_loop 2261..2609 /label=miRNA FF4 misc_feature 2610..2630 /label=splice site 2 CDS 353..2630 /label=BBa_J85800 misc_feature 1..2630 /label=BBa_J85900 misc_feature 2631..2675 /label=scar 2 terminator 2631..2675 /label=BBa_J85602 promoter 2691..3965 /label=Hef1a misc_feature 2891..2911 /label=lacO1 misc_feature 2962..2981 /label=lacOid promoter 2676..3990 /label=BBa_J85702 misc_feature 3992..4012 /label=attB1 misc_feature 3991..4012 /label=BBa_J85600 CDS 4048..4767 /label=EYFP stem_loop 4779..4800 /label=FF4 stem_loop 4801..4822 /label=FF4 stem_loop 4823..4844 /label=FF4 stem_loop 4845..4866 /label=FF4 CDS 4013..4872 /label=BBa_J85802 misc_feature 2676..4872 /label=BBa_J85902 misc_feature 4873..4917 /label=scar 3 terminator 4873..4917 /label=BBa_J85603 promoter 4940..6113 /label=pHef1A promoter 4918..6135 /label=BBa_J85701 misc_feature 6137..6157 /label=attB1 misc_feature 6136..6157 /label=BBa_J85600 CDS 6167..6871 /label=rtTA3 misc_feature 6878..6931 /label=2A misc_feature 6938..6961 /label=NLS CDS 6962..7657 /label=mKate2 CDS 6158..7661 /label=BBa_J85801 misc_feature 4918..7661 /label=BBa_J85901 ORIGIN 1 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag 61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct 121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg 181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag 241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg 301 aaccgtcaga tcgcaaaggg cgaattcgac ccaagtttgt acaaaaaagc agtcccacca 361 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt 421 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg 481 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt 541 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg 601 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac 661 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg 721 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca 781 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt 841 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc 901 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct 961 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga 1021 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg 1081 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg 1141 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat 1201 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg 1261 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg 1321 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg 1381 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcaga 1441 gcagcctgag gcctcctaag aagaagagga aggttgaggg ccgcggcagc ctgctgacct 1501 gcggcgacgt ggaggaaaac cccggcccca tggtgagcaa gggcgaggag ctgttcaccg 1561 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtga 1621 ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca 1681 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagccac ggcgtgcagt 1741 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 1801 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcacctac aagacccgcg 1861 ccgaggtgaa gttcgagggc gacaccctag tgaaccgcat cgagctgaag ggcgtcgact 1921 tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac agccacaaca 1981 tctatatcat ggccgtcaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 2041 acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 2101 acggccccgt gctgctgccc gacagccact acctgagcac ccagtccgtg ctgagcaaag 2161 accccaacga gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc gccgggatca 2221 ctctcggcat ggacgagctg tacaagtaag aggtgagtat gtgctcgctt cggcagcaca 2281 tatactatgt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa 2341 cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagtgc tgttgacagt 2401 gagcgccgct tgaagttttt aattaaatag tgaagccaca gatgtattta attaaagact 2461 tcaagcggtg cctactgcct cggagaattc aaggggctac tttaggagca attatcttgt 2521 ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat 2581 ggtataaatt aaatcacttt tttcaattgt ttcctttttt ttcctcaggg tttaccgcat 2641 ctagacctaa ctgagatact gccatagacg actacgctcc gaattctaat tggctccggt 2701 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 2761 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 2821 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 2881 gtgaagctag aattgtgagc ggataacaat tccacagtcg accctaggtt gtgtcgcgag 2941 tgttggatcc cagctgacac caattgtgag cgctcacaat tgctagccgt tctttttcgc 3001 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 3061 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt 3121 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 3181 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 3241 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 3301 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 3361 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 3421 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 3481 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 3541 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 3601 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3661 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3721 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3781 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3841 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3901 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3961 caggtgaaga cttgatcccc ggaattcgac ccaagtttgt acaaaaaagc agttcttcca 4021 tttcaggtgg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg 4081 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 4141 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 4201 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc 4261 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 4321 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 4381 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 4441 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 4501 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac 4561 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 4621 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaagac 4681 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 4741 ctcggcatgg acgagctgta caagtaaagc ggcgcgcccc gcttgaagtc tttaattaaa 4801 ccgcttgaag tctttaatta aaccgcttga agtctttaat taaaccgctt gaagtcttta 4861 attaaagcta gtccatccct ctgggcctta gatagccgga tacagtgact ttgaaaggct 4921 ccgaattcgc ccttattaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc 4981 acagtccccg agaagttgtg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg 5041 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 5101 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 5161 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat 5221 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag 5281 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct 5341 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac 5401 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct 5461 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact 5521 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt 5581 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct 5641 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca 5701 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct 5761 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca 5821 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg 5881 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg 5941 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg 6001 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg 6061 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtggatccc 6121 aagggcgaat tcgacccaag tttgtacaaa aaagcaggac gccaccatgt ctaggctgga 6181 caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct 6241 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt 6301 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac 6361 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc 6421 ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc 6481 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt 6541 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt 6601 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc 6661 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc 6721 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag 6781 cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct 6841 tgacgatttt gaccttgaca tgctccccgg ggaattggag ggccgcggca gcctgctgac 6901 ctgcggcgac gtggaggaaa accccggccc catggtgcca ccaaaaaaaa aacgcaaagt 6961 tgtgagcgag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa 7021 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac 7081 catgagaatc aaggcggtcg agggcggccc tctccccttc gccttcgaca tcctggctac 7141 cagcttcatg tacggcagca aaaccttcat caaccacacc cagggcatcc ccgacttctt 7201 taagcagtcc ttccccgagg gcttcacatg ggagagagtc accacatacg aagacggggg 7261 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa 7321 gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg 7381 ggaggcctcc accgagaccc tgtaccccgc tgacggcggc ctggaaggca gagccgacat 7441 ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttgaagacca catacagatc 7501 caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggaca gaagactgga 7561 aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag 7621 atactgcgac ctccctagca aactggggca cagatgatac c //