LOCUS       BBa_J90002              1018 bp    DNA     linear       14-Jun-2024
DEFINITION  BCP reporter
ACCESSION   BBa_J90002
VERSION     BBa_J90002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             221..886
                     /label=amilCP
     CDS             221..889
                     /label=BBa_K592009
     stem_loop       901..944
                     /label=stem_loop
     misc_feature    890..969
                     /label=BBa_B0010
     terminator      890..969
                     /label=BBa_B0010
     stem_loop       985..1004
                     /label=T7 TE
     misc_feature    1011..1011
                     /label=stop
     polyA_site      1005..1018
                     /label=polya
     misc_feature    978..1018
                     /label=BBa_B0012
     terminator      978..1018
                     /label=BBa_B0012
     terminator      890..1018
                     /label=BBa_B0015
     misc_feature    209..1018
                     /label=BBa_J90001
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa atgagtgtga tcgctaaaca
      241 aatgacctac aaggtttata tgtcaggcac ggtcaatgga cactactttg aggtcgaagg
      301 cgatggaaaa ggtaagccct acgaggggga gcagacggta aagctcactg tcaccaaggg
      361 cggacctctg ccatttgctt gggatatttt atcaccacag tgtcagtacg gaagcatacc
      421 attcaccaag taccctgaag acatccctga ctatgtaaag cagtcattcc cggagggcta
      481 tacatgggag aggatcatga actttgaaga tggtgcagtg tgtactgtca gcaatgattc
      541 cagcatccaa ggcaactgtt tcatctacca tgtcaagttc tctggtttga actttcctcc
      601 caatggacct gtcatgcaga agaagacaca gggctgggaa cccaacactg agcgtctctt
      661 tgcacgagat ggaatgctgc taggaaacaa ctttatggct ctgaagttag aaggaggcgg
      721 tcactatttg tgtgaattta aaactactta caaggcaaag aagcctgtga agatgccagg
      781 gtatcactat gttgaccgca aactggatgt aaccaatcac aacaaggatt acacttcggt
      841 tgagcagtgt gaaatttcca ttgcacgcaa acctgtggtc gcctaataac caggcatcaa
      901 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      961 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//