LOCUS       BBa_J95003              2884 bp    DNA     linear       18-May-2024
DEFINITION  Bioluminescence based Promoter characterization Kit R
ACCESSION   BBa_J95003
VERSION     BBa_J95003.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    20..955
                     /label=BBa_J95001
     stem_loop       971..990
                     /label=T7 TE
     misc_feature    997..997
                     /label=stop
     polyA_site      991..1004
                     /label=polya
     misc_feature    964..1004
                     /label=BBa_B0012
     terminator      964..1004
                     /label=BBa_B0012
     stem_loop       1025..1047
                     /label=stem_loop
     misc_feature    1013..1058
                     /label=BBa_B0011
     terminator      1013..1058
                     /label=BBa_B0011
     promoter        1067..1101
                     /label=BBa_J23101
     RBS             1110..1122
                     /label=RBS-3\Weak
     RBS             1116..1119
                     /label=RBS
     misc_feature    1110..1122
                     /label=BBa_B0032
     RBS             1110..1122
                     /label=BBa_B0032
     misc_feature    1129..2781
                     /label=BBa_J95000
     stem_loop       2797..2816
                     /label=T7 TE
     misc_feature    2823..2823
                     /label=stop
     polyA_site      2817..2830
                     /label=polya
     misc_feature    2790..2830
                     /label=BBa_B0012
     terminator      2790..2830
                     /label=BBa_B0012
     stem_loop       2851..2873
                     /label=stem_loop
     misc_feature    2839..2884
                     /label=BBa_B0011
     terminator      2839..2884
                     /label=BBa_B0011
ORIGIN
        1 tcacacagga aagtactaga tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat
       61 gatcactggg cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat
      121 caactactat gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc
      181 tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat
      241 catccctgat ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct
      301 cctggatcac tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat
      361 catctttgtg ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca
      421 agacaagatc aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga
      481 cgagtggcct gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat
      541 ggtgcttgag aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact
      601 ggagcctgag gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg
      661 gcctaccctc tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt
      721 ccagattgtc cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt
      781 catcgagtcc gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc
      841 taacaccgag ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga
      901 aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agtaatacta
      961 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
     1021 taaaaagcca gattattaat ccggcttttt tattatttta ctagagttta cagctagctc
     1081 agtcctaggt attatgctag ctactagagt cacacaggaa agtactagat ggaagacgcc
     1141 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
     1201 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     1261 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
     1321 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     1381 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     1441 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
     1501 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
     1561 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     1621 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
     1681 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     1741 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     1801 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     1861 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     1921 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     1981 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     2041 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     2101 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     2161 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     2221 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     2281 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     2341 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     2401 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     2461 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     2521 ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     2581 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     2641 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     2701 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     2761 ggcggaaaga tcgccgtgta atactagagt cacactggct caccttcggg tgggcctttc
     2821 tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg cttttttatt
     2881 attt
//