LOCUS       BBa_J95004              2865 bp    DNA     linear       21-May-2024
DEFINITION  Bioluminescence based RBS characterization kit F
ACCESSION   BBa_J95004
VERSION     BBa_J95004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1653
                     /label=BBa_J95000
     stem_loop       1669..1688
                     /label=T7 TE
     misc_feature    1695..1695
                     /label=stop
     polyA_site      1689..1702
                     /label=polya
     misc_feature    1662..1702
                     /label=BBa_B0012
     terminator      1662..1702
                     /label=BBa_B0012
     stem_loop       1723..1745
                     /label=stem_loop
     misc_feature    1711..1756
                     /label=BBa_B0011
     terminator      1711..1756
                     /label=BBa_B0011
     promoter        1765..1799
                     /label=BBa_J23101
     RBS             1808..1820
                     /label=RBS-3\Weak
     RBS             1814..1817
                     /label=RBS
     misc_feature    1808..1820
                     /label=BBa_B0032
     RBS             1808..1820
                     /label=BBa_B0032
     misc_feature    1827..2762
                     /label=BBa_J95001
     stem_loop       2778..2797
                     /label=T7 TE
     misc_feature    2804..2804
                     /label=stop
     polyA_site      2798..2811
                     /label=polya
     misc_feature    2771..2811
                     /label=BBa_B0012
     terminator      2771..2811
                     /label=BBa_B0012
     stem_loop       2832..2854
                     /label=stem_loop
     misc_feature    2820..2865
                     /label=BBa_B0011
     terminator      2820..2865
                     /label=BBa_B0011
ORIGIN
        1 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
       61 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      121 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
      181 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      241 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      301 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
      361 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
      421 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
      481 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
      541 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
      601 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
      661 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
      721 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
      781 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
      841 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
      901 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
      961 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     1021 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1081 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1141 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     1201 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1261 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     1321 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     1381 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     1441 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     1501 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     1561 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     1621 aaggccaaga agggcggaaa gatcgccgtg taatactaga gtcacactgg ctcaccttcg
     1681 ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc
     1741 ggctttttta ttattttact agagtttaca gctagctcag tcctaggtat tatgctagct
     1801 actagagtca cacaggaaag tactagatgg cttccaaggt gtacgacccc gagcaacgca
     1861 aacgcatgat cactgggcct cagtggtggg ctcgctgcaa gcaaatgaac gtgctggact
     1921 ccttcatcaa ctactatgat tccgagaagc acgccgagaa cgccgtgatt tttctgcatg
     1981 gtaacgctgc ctccagctac ctgtggaggc acgtcgtgcc tcacatcgag cccgtggcta
     2041 gatgcatcat ccctgatctg atcggaatgg gtaagtccgg caagagcggg aatggctcat
     2101 atcgcctcct ggatcactac aagtacctca ccgcttggtt cgagctgctg aaccttccaa
     2161 agaaaatcat ctttgtgggc cacgactggg gggcttgtct ggcctttcac tactcctacg
     2221 agcaccaaga caagatcaag gccatcgtcc atgctgagag tgtcgtggac gtgatcgagt
     2281 cctgggacga gtggcctgac atcgaggagg atatcgccct gatcaagagc gaagagggcg
     2341 agaaaatggt gcttgagaat aacttcttcg tcgagaccat gctcccaagc aagatcatgc
     2401 ggaaactgga gcctgaggag ttcgctgcct acctggagcc attcaaggag aagggcgagg
     2461 ttagacggcc taccctctcc tggcctcgcg agatccctct cgttaaggga ggcaagcccg
     2521 acgtcgtcca gattgtccgc aactacaacg cctaccttcg ggccagcgac gatctgccta
     2581 agatgttcat cgagtccgac cctgggttct tttccaacgc tattgtcgag ggagctaaga
     2641 agttccctaa caccgagttc gtgaaggtga agggcctcca cttcagccag gaggacgctc
     2701 cagatgaaat gggtaagtac atcaagagct tcgtggagcg cgtgctgaag aacgagcagt
     2761 aatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga
     2821 gagaatataa aaagccagat tattaatccg gcttttttat tattt
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