LOCUS       BBa_J95005              2865 bp    DNA     linear       21-May-2024
DEFINITION  One of two Bioluminescence based BioBrick RBS characterization kits
ACCESSION   BBa_J95005
VERSION     BBa_J95005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..936
                     /label=BBa_J95001
     stem_loop       952..971
                     /label=T7 TE
     misc_feature    978..978
                     /label=stop
     polyA_site      972..985
                     /label=polya
     misc_feature    945..985
                     /label=BBa_B0012
     terminator      945..985
                     /label=BBa_B0012
     stem_loop       1006..1028
                     /label=stem_loop
     misc_feature    994..1039
                     /label=BBa_B0011
     terminator      994..1039
                     /label=BBa_B0011
     promoter        1048..1082
                     /label=BBa_J23101
     RBS             1091..1103
                     /label=RBS-3\Weak
     RBS             1097..1100
                     /label=RBS
     misc_feature    1091..1103
                     /label=BBa_B0032
     RBS             1091..1103
                     /label=BBa_B0032
     misc_feature    1110..2762
                     /label=BBa_J95000
     stem_loop       2778..2797
                     /label=T7 TE
     misc_feature    2804..2804
                     /label=stop
     polyA_site      2798..2811
                     /label=polya
     misc_feature    2771..2811
                     /label=BBa_B0012
     terminator      2771..2811
                     /label=BBa_B0012
     stem_loop       2832..2854
                     /label=stem_loop
     misc_feature    2820..2865
                     /label=BBa_B0011
     terminator      2820..2865
                     /label=BBa_B0011
ORIGIN
        1 atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg
       61 tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag
      121 aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg
      181 aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga
      241 atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac
      301 ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac
      361 tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc
      421 gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag
      481 gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc
      541 ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct
      601 gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct
      661 cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac
      721 aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg
      781 ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag
      841 gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag
      901 agcttcgtgg agcgcgtgct gaagaacgag cagtaatact agagtcacac tggctcacct
      961 tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa
     1021 tccggctttt ttattatttt actagagttt acagctagct cagtcctagg tattatgcta
     1081 gctactagag tcacacagga aagtactaga tggaagacgc caaaaacata aagaaaggcc
     1141 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
     1201 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
     1261 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
     1321 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
     1381 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
     1441 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
     1501 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
     1561 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
     1621 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga
     1681 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc
     1741 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa
     1801 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
     1861 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
     1921 agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc
     1981 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
     2041 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt
     2101 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga
     2161 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
     2221 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt
     2281 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
     2341 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
     2401 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
     2461 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
     2521 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     2581 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     2641 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     2701 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt
     2761 aatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga
     2821 gagaatataa aaagccagat tattaatccg gcttttttat tattt
//