LOCUS       BBa_J95006              2884 bp    DNA     linear       18-May-2024
DEFINITION  One of two BioBrick terminator characterization kits
ACCESSION   BBa_J95006
VERSION     BBa_J95006.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    63..1715
                     /label=BBa_J95000
     stem_loop       1731..1750
                     /label=T7 TE
     misc_feature    1757..1757
                     /label=stop
     polyA_site      1751..1764
                     /label=polya
     misc_feature    1724..1764
                     /label=BBa_B0012
     terminator      1724..1764
                     /label=BBa_B0012
     stem_loop       1785..1807
                     /label=stem_loop
     misc_feature    1773..1818
                     /label=BBa_B0011
     terminator      1773..1818
                     /label=BBa_B0011
     RBS             1827..1839
                     /label=RBS-3\Weak
     RBS             1833..1836
                     /label=RBS
     misc_feature    1827..1839
                     /label=BBa_B0032
     RBS             1827..1839
                     /label=BBa_B0032
     misc_feature    1846..2781
                     /label=BBa_J95001
     stem_loop       2797..2816
                     /label=T7 TE
     misc_feature    2823..2823
                     /label=stop
     polyA_site      2817..2830
                     /label=polya
     misc_feature    2790..2830
                     /label=BBa_B0012
     terminator      2790..2830
                     /label=BBa_B0012
     stem_loop       2851..2873
                     /label=stem_loop
     misc_feature    2839..2884
                     /label=BBa_B0011
     terminator      2839..2884
                     /label=BBa_B0011
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
      121 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
      181 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
      241 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
      301 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
      361 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
      421 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
      481 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
      541 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
      601 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
      661 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
      721 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
      781 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
      841 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
      901 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
      961 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     1021 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     1081 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     1141 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     1201 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     1261 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     1321 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     1381 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     1441 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     1501 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     1561 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     1621 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     1681 taaaggccaa gaagggcgga aagatcgccg tgtaatacta gagtcacact ggctcacctt
     1741 cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat
     1801 ccggcttttt tattatttta ctagagtcac acaggaaagt actagatggc ttccaaggtg
     1861 tacgaccccg agcaacgcaa acgcatgatc actgggcctc agtggtgggc tcgctgcaag
     1921 caaatgaacg tgctggactc cttcatcaac tactatgatt ccgagaagca cgccgagaac
     1981 gccgtgattt ttctgcatgg taacgctgcc tccagctacc tgtggaggca cgtcgtgcct
     2041 cacatcgagc ccgtggctag atgcatcatc cctgatctga tcggaatggg taagtccggc
     2101 aagagcggga atggctcata tcgcctcctg gatcactaca agtacctcac cgcttggttc
     2161 gagctgctga accttccaaa gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg
     2221 gcctttcact actcctacga gcaccaagac aagatcaagg ccatcgtcca tgctgagagt
     2281 gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca tcgaggagga tatcgccctg
     2341 atcaagagcg aagagggcga gaaaatggtg cttgagaata acttcttcgt cgagaccatg
     2401 ctcccaagca agatcatgcg gaaactggag cctgaggagt tcgctgccta cctggagcca
     2461 ttcaaggaga agggcgaggt tagacggcct accctctcct ggcctcgcga gatccctctc
     2521 gttaagggag gcaagcccga cgtcgtccag attgtccgca actacaacgc ctaccttcgg
     2581 gccagcgacg atctgcctaa gatgttcatc gagtccgacc ctgggttctt ttccaacgct
     2641 attgtcgagg gagctaagaa gttccctaac accgagttcg tgaaggtgaa gggcctccac
     2701 ttcagccagg aggacgctcc agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc
     2761 gtgctgaaga acgagcagta atactagagt cacactggct caccttcggg tgggcctttc
     2821 tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg cttttttatt
     2881 attt
//