LOCUS       BBa_J95007              2884 bp    DNA     linear       18-May-2024
DEFINITION  One of two BioBrick terminator characterization kits
ACCESSION   BBa_J95007
VERSION     BBa_J95007.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    63..998
                     /label=BBa_J95001
     stem_loop       1014..1033
                     /label=T7 TE
     misc_feature    1040..1040
                     /label=stop
     polyA_site      1034..1047
                     /label=polya
     misc_feature    1007..1047
                     /label=BBa_B0012
     terminator      1007..1047
                     /label=BBa_B0012
     stem_loop       1068..1090
                     /label=stem_loop
     misc_feature    1056..1101
                     /label=BBa_B0011
     terminator      1056..1101
                     /label=BBa_B0011
     RBS             1110..1122
                     /label=RBS-3\Weak
     RBS             1116..1119
                     /label=RBS
     misc_feature    1110..1122
                     /label=BBa_B0032
     RBS             1110..1122
                     /label=BBa_B0032
     misc_feature    1129..2781
                     /label=BBa_J95000
     stem_loop       2797..2816
                     /label=T7 TE
     misc_feature    2823..2823
                     /label=stop
     polyA_site      2817..2830
                     /label=polya
     misc_feature    2790..2830
                     /label=BBa_B0012
     terminator      2790..2830
                     /label=BBa_B0012
     stem_loop       2851..2873
                     /label=stem_loop
     misc_feature    2839..2884
                     /label=BBa_B0011
     terminator      2839..2884
                     /label=BBa_B0011
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggcttc caaggtgtac gaccccgagc aacgcaaacg catgatcact gggcctcagt
      121 ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac tatgattccg
      181 agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc agctacctgt
      241 ggaggcacgt cgtgcctcac atcgagcccg tggctagatg catcatccct gatctgatcg
      301 gaatgggtaa gtccggcaag agcgggaatg gctcatatcg cctcctggat cactacaagt
      361 acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg
      421 actggggggc ttgtctggcc tttcactact cctacgagca ccaagacaag atcaaggcca
      481 tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg
      541 aggaggatat cgccctgatc aagagcgaag agggcgagaa aatggtgctt gagaataact
      601 tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa actggagcct gaggagttcg
      661 ctgcctacct ggagccattc aaggagaagg gcgaggttag acggcctacc ctctcctggc
      721 ctcgcgagat ccctctcgtt aagggaggca agcccgacgt cgtccagatt gtccgcaact
      781 acaacgccta ccttcgggcc agcgacgatc tgcctaagat gttcatcgag tccgaccctg
      841 ggttcttttc caacgctatt gtcgagggag ctaagaagtt ccctaacacc gagttcgtga
      901 aggtgaaggg cctccacttc agccaggagg acgctccaga tgaaatgggt aagtacatca
      961 agagcttcgt ggagcgcgtg ctgaagaacg agcagtaata ctagagtcac actggctcac
     1021 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
     1081 aatccggctt ttttattatt ttactagagt cacacaggaa agtactagat ggaagacgcc
     1141 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
     1201 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     1261 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
     1321 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     1381 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     1441 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
     1501 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
     1561 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     1621 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
     1681 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     1741 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     1801 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     1861 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     1921 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     1981 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     2041 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     2101 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     2161 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     2221 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     2281 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     2341 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     2401 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     2461 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     2521 ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     2581 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     2641 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     2701 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     2761 ggcggaaaga tcgccgtgta atactagagt cacactggct caccttcggg tgggcctttc
     2821 tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg cttttttatt
     2881 attt
//