LOCUS       BBa_K077014             1766 bp    DNA     linear       16-May-2024
DEFINITION  ptet with LuxR and LasR
ACCESSION   BBa_K077014
VERSION     BBa_K077014.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..621
                     /label=LasI
     misc_feature    622..654
                     /label=LVA
     misc_feature    661..685
                     /label=Help:Barcodes
     CDS             19..660
                     /label=BBa_C0078
     stem_loop       705..748
                     /label=stem_loop
     misc_feature    694..773
                     /label=BBa_B0010
     terminator      694..773
                     /label=BBa_B0010
     stem_loop       789..808
                     /label=T7 TE
     misc_feature    815..815
                     /label=stop
     polyA_site      809..822
                     /label=polya
     misc_feature    782..822
                     /label=BBa_B0012
     terminator      782..822
                     /label=BBa_B0012
     misc_feature    835..838
                     /label=conserved
     RBS             831..842
                     /label=BBa_B0034
     CDS             849..1565
                     /label=lasR
     misc_feature    1566..1598
                     /label=LVA
     misc_feature    1328..1328
                     /label=G
     misc_feature    1605..1629
                     /label=Help:Barcodes
     CDS             849..1604
                     /label=BBa_C0079
     stem_loop       1649..1692
                     /label=stem_loop
     misc_feature    1638..1717
                     /label=BBa_B0010
     terminator      1638..1717
                     /label=BBa_B0010
     stem_loop       1733..1752
                     /label=T7 TE
     misc_feature    1759..1759
                     /label=stop
     polyA_site      1753..1766
                     /label=polya
     misc_feature    1726..1766
                     /label=BBa_B0012
     terminator      1726..1766
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gatcgttcag atcggtcgtc gtgaagagtt cgacaaaaaa
       61 ctgctgggtg aaatgcacaa actgcgtgct caggttttca aagaacgtaa aggttgggac
      121 gtttccgtta tcgacgaaat ggaaatcgac ggttacgacg ctctgtcccc gtactacatg
      181 ctgatccagg aagacacccc ggaagctcag gttttcggtt gctggcgtat cttcgacacc
      241 accggtccgt acatgctgaa aaacaccttc ccggaactgc tgcacggtaa agaagctccg
      301 tgctccccgc acatctggga actgtcccgt ttcgctatca actccggtca gaaaggttcc
      361 ctgggtttct ccgactgcac cctggaagct atgcgtgctc tggctcgtta ctccttgcag
      421 aacgacatcc agaccctggt taccgttacc accgttggtg ttgaaaaaat gatgatccgt
      481 gctggtctgg acgtttcccg tttcggtccg cacctgaaaa tcggtatcga acgtgctgtt
      541 gctctgcgta tcgaactgaa cgctaaaacc cagatcgctc tgtacggtgg tgttctggtt
      601 gaacagcgtc tggctgtttc cgctgctaac gacgaaaact acgctctggt tgcttaataa
      661 ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc
      721 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      781 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag aaagaggaga
      841 aatactagat ggccttggtt gacggttttc ttgagctgga acgctcaagt ggaaaattgg
      901 agtggagcgc catcctccag aagatggcga gcgaccttgg attctcgaag atcctgttcg
      961 gcctgttgcc taaggacagc caggactacg agaacgcctt catcgtcggc aactacccgg
     1021 ccgcctggcg cgagcattac gaccgggctg gctacgcgcg ggtcgacccg acggtcagtc
     1081 actgtaccca gagcgtactg ccgattttct gggaaccgtc catctaccag acgcgaaagc
     1141 agcacgagtt cttcgaggaa gcctcggccg ccggcctggt gtatgggctg accatgccgc
     1201 tgcatggtgc tcgcggcgaa ctcggcgcgc tgagcctcag cgtggaagcg gaaaaccggg
     1261 ccgaggccaa ccgtttcata gagtcggtcc tgccgaccct gtggatgctc aaggactacg
     1321 cactgcaaag cggtgccgga ctggccttcg aacatccggt cagcaaaccg gtggttctga
     1381 ccagccggga gaaggaagtg ttgcagtggt gcgccatcgg caagaccagt tgggagatat
     1441 cggttatctg caactgctcg gaagccaatg tgaacttcca tatgggaaat attcggcgga
     1501 agttcggtgt gacctcccgc cgcgtagcgg ccattatggc cgttaatttg ggtcttatta
     1561 ctctcgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt
     1621 gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1681 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1741 ttcgggtggg cctttctgcg tttata
//