LOCUS       BBa_K077026             2655 bp    DNA     linear       13-May-2024
DEFINITION  HSL Receiver device and sender.
ACCESSION   BBa_K077026
VERSION     BBa_K077026.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     misc_feature    1007..1103
                     /label=BBa_R0065
     misc_feature    1012..1031
                     /label=Lux Box
     promoter        1053..1058
                     /label=-10
     misc_feature    1063..1079
                     /label=OR2 cI
     promoter        1064..1064
                     /label=lux p(R) start
     promoter        1077..1082
                     /label=-35
     misc_feature    1087..1103
                     /label=OR1 cI
     promoter        1100..1103
                     /label=-10
     promoter        1007..1103
                     /label=BBa_R0065
     misc_feature    1116..1119
                     /label=conserved
     RBS             1112..1123
                     /label=BBa_B0034
     misc_feature    1709..1740
                     /label=LVA
     CDS             1130..1708
                     /label=luxI
     misc_feature    1130..1747
                     /label=BBa_C0061
     misc_feature    1748..1772
                     /label=Help:Barcodes
     CDS             1130..1747
                     /label=BBa_C0061
     misc_feature    1785..1788
                     /label=conserved
     RBS             1781..1792
                     /label=BBa_B0034
     CDS             1799..2518
                     /label=GFP protein
     CDS             1799..2518
                     /label=BBa_E0040
     stem_loop       2538..2581
                     /label=stem_loop
     misc_feature    2527..2606
                     /label=BBa_B0010
     terminator      2527..2606
                     /label=BBa_B0010
     stem_loop       2622..2641
                     /label=T7 TE
     misc_feature    2648..2648
                     /label=stop
     polyA_site      2642..2655
                     /label=polya
     misc_feature    2615..2655
                     /label=BBa_B0012
     terminator      2615..2655
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaag cacctgtagg
     1021 atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataacaccg tgcgtgttga
     1081 ctattttacc tctggcggtg atatactaga gaaagaggag aaatactaga tgactataat
     1141 gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct
     1201 tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga
     1261 atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa
     1321 tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt
     1381 tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg
     1441 ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa
     1501 actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt
     1561 aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg
     1621 agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa
     1681 tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc
     1741 ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga aatactagat
     1801 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1861 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     1921 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     1981 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     2041 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     2101 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     2161 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     2221 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     2281 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     2341 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     2401 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     2461 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata
     2521 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2581 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2641 ctttctgcgt ttata
//