LOCUS       BBa_K077038             1547 bp    DNA     linear       11-May-2024
DEFINITION  HSL enzyme together with GFP
ACCESSION   BBa_K077038
VERSION     BBa_K077038.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    598..629
                     /label=LVA
     CDS             19..597
                     /label=luxI
     misc_feature    19..636
                     /label=BBa_C0061
     misc_feature    637..661
                     /label=Help:Barcodes
     CDS             19..636
                     /label=BBa_C0061
     misc_feature    1..661
                     /label=BBa_C0261
     RBS             670..684
                     /label=RBS-1\Strong
     RBS             677..681
                     /label=RBS
     misc_feature    670..684
                     /label=BBa_B0030
     RBS             670..684
                     /label=BBa_B0030
     CDS             691..1410
                     /label=GFP protein
     CDS             691..1410
                     /label=BBa_E0040
     stem_loop       1430..1473
                     /label=stem_loop
     misc_feature    1419..1498
                     /label=BBa_B0010
     terminator      1419..1498
                     /label=BBa_B0010
     stem_loop       1514..1533
                     /label=T7 TE
     misc_feature    1540..1540
                     /label=stop
     polyA_site      1534..1547
                     /label=polya
     misc_feature    1507..1547
                     /label=BBa_B0012
     terminator      1507..1547
                     /label=BBa_B0012
     misc_feature    670..1547
                     /label=BBa_E0840
ORIGIN
        1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca
       61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag
      121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa
      181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca
      241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc
      301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata
      361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt
      421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag
      481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact
      541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct
      601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct
      661 ctactagaga ttaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga
      721 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      781 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      841 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      901 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      961 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
     1021 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     1081 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     1141 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
     1201 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     1261 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     1321 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     1381 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
     1441 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1501 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//