LOCUS       BBa_K077048             1864 bp    DNA     linear       17-May-2024
DEFINITION  Testing part BBa_R0062, by GFP output.
ACCESSION   BBa_K077048
VERSION     BBa_K077048.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             83..802
                     /label=GFP protein
     CDS             83..802
                     /label=BBa_E0040
     terminator      811..858
                     /label=BBa_B0016
     misc_feature    867..885
                     /label=TetR 1
     misc_feature    867..920
                     /label=BBa_R0040
     promoter        886..891
                     /label=-35
     misc_feature    892..910
                     /label=TetR 2
     promoter        909..914
                     /label=-10
     promoter        867..920
                     /label=BBa_R0040
     misc_feature    933..936
                     /label=conserved
     RBS             929..940
                     /label=BBa_B0034
     misc_feature    947..948
                     /label=prefix
     misc_feature    1120..1120
                     /label=T
     misc_feature    1438..1438
                     /label=A
     CDS             947..1696
                     /label=luxR
     misc_feature    947..1702
                     /label=BBa_C0062
     misc_feature    1703..1727
                     /label=Help:Barcodes
     CDS             947..1702
                     /label=BBa_C0062
     stem_loop       1747..1790
                     /label=stem_loop
     misc_feature    1736..1815
                     /label=BBa_B0010
     terminator      1736..1815
                     /label=BBa_B0010
     stem_loop       1831..1850
                     /label=T7 TE
     misc_feature    1857..1857
                     /label=stop
     polyA_site      1851..1864
                     /label=polya
     misc_feature    1824..1864
                     /label=BBa_B0012
     terminator      1824..1864
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagtcacaca ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      121 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      181 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      241 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      301 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      361 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      421 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      481 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      541 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      601 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      661 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      721 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
      781 ggatgaacta tacaaataat aatactagag ctagcataac cccttggggc ctctaaacgg
      841 gtcttgaggg gttttttgta ctagagtccc tatcagtgat agagattgac atccctatca
      901 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatga aaaacataaa
      961 tgccgacgac acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat
     1021 taatcaatgc ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat
     1081 catttatcct cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa
     1141 atggaggcaa tattatgatg acgctaattt aataaaatat gatcctatag tagattattc
     1201 taactccaat cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa
     1261 atctccaaat gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc
     1321 tattcatacg gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa
     1381 ctatatagat agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct
     1441 agttgataat tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa
     1501 aagagaaaaa gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa
     1561 aatattaggt tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact
     1621 caatacaaca aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg
     1681 cccatacttt aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg
     1741 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1801 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1861 tata
//