LOCUS       BBa_K077050              935 bp    DNA     linear       17-May-2024
DEFINITION  Testing pcI with GFP output.
ACCESSION   BBa_K077050
VERSION     BBa_K077050.1  
FEATURES             Location/Qualifiers
     promoter        15..20
                     /label=-35
     promoter        25..41
                     /label=OR1
     promoter        1..17
                     /label=OR2
     promoter        38..43
                     /label=-10
     misc_feature    1..49
                     /label=BBa_R1051
     promoter        1..49
                     /label=BBa_R1051
     RBS             58..72
                     /label=RBS-1\Strong
     RBS             65..69
                     /label=RBS
     misc_feature    58..72
                     /label=BBa_B0030
     RBS             58..72
                     /label=BBa_B0030
     CDS             79..798
                     /label=GFP protein
     CDS             79..798
                     /label=BBa_E0040
     stem_loop       818..861
                     /label=stem_loop
     misc_feature    807..886
                     /label=BBa_B0010
     terminator      807..886
                     /label=BBa_B0010
     stem_loop       902..921
                     /label=T7 TE
     misc_feature    928..928
                     /label=stop
     polyA_site      922..935
                     /label=polya
     misc_feature    895..935
                     /label=BBa_B0012
     terminator      895..935
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgaca attttacctc tggcggtgat actggttgct actagagatt
       61 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
      121 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      181 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      241 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      301 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      361 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      421 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      481 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      541 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      601 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      661 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      721 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      781 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      901 tggctcacct tcgggtgggc ctttctgcgt ttata
//