LOCUS       BBa_K079003             2093 bp    DNA     linear       17-May-2024
DEFINITION  LacI protein with RBS and GFP reporter protein
ACCESSION   BBa_K079003
VERSION     BBa_K079003.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     misc_feature    1184..1187
                     /label=conserved
     RBS             1180..1191
                     /label=BBa_B0034
     misc_feature    1198..1917
                     /label=BBa_E0040
     misc_feature    1912..1950
                     /label=LVA
     misc_feature    1951..1956
                     /label=Double Stop
     misc_feature    1198..1200
                     /label=Start
     misc_feature    1198..1956
                     /label=BBa_J04031
     stem_loop       1976..2019
                     /label=stem_loop
     misc_feature    1965..2044
                     /label=BBa_B0010
     terminator      1965..2044
                     /label=BBa_B0010
     stem_loop       2060..2079
                     /label=T7 TE
     misc_feature    2086..2086
                     /label=stop
     polyA_site      2080..2093
                     /label=polya
     misc_feature    2053..2093
                     /label=BBa_B0012
     terminator      2053..2093
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagaga aagaggagaa atactagatg
     1201 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1261 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1321 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     1381 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
     1441 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1501 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     1561 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     1621 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1681 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     1741 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1801 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     1861 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa aaggcctgct
     1921 gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa
     1981 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2041 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//