LOCUS       BBa_K084007              835 bp    DNA     linear       06-May-2024
DEFINITION  Lac repressible lasI generator (No LVA )
ACCESSION   BBa_K084007
VERSION     BBa_K084007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..835
                     /label=lasI
     CDS             227..835
                     /label=BBa_C0178
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga tcgttcagat
      241 cggtcgtcgt gaagagttcg acaaaaaact gctgggtgaa atgcacaaac tgcgtgctca
      301 ggttttcaaa gaacgtaaag gttgggacgt ttccgttatc gacgaaatgg aaatcgacgg
      361 ttacgacgct ctgtccccgt actacatgct gatccaggaa gacaccccgg aagctcaggt
      421 tttcggttgc tggcgtatct tcgacaccac cggtccgtac atgctgaaaa acaccttccc
      481 ggaactgctg cacggtaaag aagctccgtg ctccccgcac atctgggaac tgtcccgttt
      541 cgctatcaac tccggtcaga aaggttccct gggtttctcc gactgcaccc tggaagctat
      601 gcgtgctctg gctcgttact ccttgcagaa cgacatccag accctggtta ccgttaccac
      661 cgttggtgtt gaaaaaatga tgatccgtgc tggtctggac gtttcccgtt tcggtccgca
      721 cctgaaaatc ggtatcgaac gtgctgttgc tctgcgtatc gaactgaacg ctaaaaccca
      781 gatcgctctg tacggtggtg ttctggttga acagcgtctg gctgtttcct aataa
//