LOCUS       BBa_K084010              953 bp    DNA     linear       20-May-2024
DEFINITION  Lac repressible CinI generator
ACCESSION   BBa_K084010
VERSION     BBa_K084010.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..889
                     /label=CinI
     misc_feature    890..922
                     /label=LVA
     misc_feature    929..953
                     /label=Help:Barcodes
     CDS             227..928
                     /label=BBa_C0076
     misc_feature    209..953
                     /label=BBa_I9030
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt tcgttatcat
      241 ccaggctcac gaataccaga aatacgctgc tgttctggac cagatgttcc gtctgcgtaa
      301 aaaagttttc gctgacaccc tgtgctggga cgttccggtt atcggtccgt acgaacgtga
      361 ctcctacgac tccctggctc cggcttacct ggtttggtgc aacgactccc gtacccgtct
      421 gtacggtggt atgcgtctga tgccgaccac cggtccgacc ctgctgtacg acgttttccg
      481 tgaaaccttc ccggacgctg ctgacctgat cgctccgggt atctgggaag gtacccgtat
      541 gtgcatcgac gaagaagcta tcgctaaaga cttcccggaa atcgacgctg gtcgtgcttt
      601 ctccatgatg ctgctggctc tgtgcgaatg cgctctggac cacggtatcc acaccatgat
      661 ctccaactac gaaccgtacc tgaaacgtgt ttacaaacgt gctggtgctg aagttgaaga
      721 actgggtcgt gctgacggtt acggtaaata cccggtttgc tgcggtgctt tcgaagtttc
      781 cgaccgtgtt ctgcgtaaaa tgcgtgctgc tctgggtctg accctgccgc tgtacgttcg
      841 tcacgttccg gctcgttccg ttgttaccca gttcctggaa atggctgctg ctgctaacga
      901 cgaaaactac gctctggttg cttaataacc ctgatagtgc tagtgtagat ccc
//