LOCUS BBa_K084011 3557 bp DNA linear 17-May-2024 DEFINITION Time device ACCESSION BBa_K084011 VERSION BBa_K084011.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23105 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..746 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 misc_feature 1..883 /label=BBa_I739001 misc_feature 892..910 /label=TetR 1 misc_feature 892..945 /label=BBa_R0040 promoter 911..916 /label=-35 misc_feature 917..935 /label=TetR 2 promoter 934..939 /label=-10 promoter 892..945 /label=BBa_R0040 misc_feature 958..961 /label=conserved RBS 954..965 /label=BBa_B0034 misc_feature 2061..2099 /label=LVA CDS 972..2099 /label=lacI-LVA misc_feature 972..2099 /label=BBa_C0012 misc_feature 2100..2124 /label=Help:Barcodes CDS 972..2099 /label=BBa_C0012 misc_feature 892..2124 /label=BBa_I730003 misc_feature 2137..2140 /label=conserved RBS 2133..2144 /label=BBa_B0034 CDS 2151..2870 /label=GFP protein CDS 2151..2870 /label=BBa_E0040 stem_loop 2890..2933 /label=stem_loop misc_feature 2879..2958 /label=BBa_B0010 terminator 2879..2958 /label=BBa_B0010 stem_loop 2974..2993 /label=T7 TE misc_feature 3000..3000 /label=stop polyA_site 2994..3007 /label=polya misc_feature 2967..3007 /label=BBa_B0012 terminator 2967..3007 /label=BBa_B0012 misc_feature 2133..3007 /label=BBa_I13504 promoter 3035..3089 /label=plac misc_feature 3035..3041 /label=-35 misc_feature 3057..3063 /label=-10 misc_feature 3042..3056 /label=O1 misc_feature 3064..3089 /label=O1 misc_feature 3016..3034 /label=luxR binding site promoter 3016..3089 /label=BBa_I751502 RBS 3098..3108 /label=RBS-4\Weaker RBS 3104..3107 /label=RBS misc_feature 3098..3108 /label=BBa_B0033 RBS 3098..3108 /label=BBa_B0033 misc_feature 3115..3117 /label=start misc_feature 3418..3420 /label=stop CDS 3115..3417 /label=cds CDS 3115..3420 /label=BBa_K145151 stem_loop 3440..3483 /label=stem_loop misc_feature 3429..3508 /label=BBa_B0010 terminator 3429..3508 /label=BBa_B0010 stem_loop 3524..3543 /label=T7 TE misc_feature 3550..3550 /label=stop polyA_site 3544..3557 /label=polya misc_feature 3517..3557 /label=BBa_B0012 terminator 3517..3557 /label=BBa_B0012 terminator 3429..3557 /label=BBa_B0015 ORIGIN 1 tttacggcta gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg 961 agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 1021 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 1081 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 1141 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 1201 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 1261 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 1321 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 1381 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 1441 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 1501 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 1561 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 1621 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 1681 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 1741 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 1801 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 1861 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 1921 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 1981 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 2041 cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac 2101 tctgatagtg ctagtgtaga tctctactag agaaagagga gaaatactag atgcgtaaag 2161 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg 2221 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc 2281 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt 2341 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt 2401 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg 2461 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 2521 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca 2581 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta 2641 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac 2701 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac 2761 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg 2821 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc 2881 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 2941 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3001 gtttatatac tagagacctg taggatcgta caggtttact tgtgagcgga taacaatata 3061 gtgtgtggaa ttgtgagcgg ataacaattt actagagtca cacaggacta ctagatgcag 3121 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt 3181 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg 3241 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc 3301 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat 3361 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa 3421 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 3481 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 3541 gcctttctgc gtttata //