BBa_K090502

BBa_K090502 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K090502

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Goodman

Date created: 2008-10-27 12:00:00

Date modified: 2015-06-09 09:03:37

Gram-Positive Xylose-Inducible Promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K090502_sequence (Version 1)

Description

This is a Xylose-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.

Notes

We used the following PCR primers to extract the part, sans biobrick ends:
Forward: TTCATGAAAAACTAAAAAAAATATTG
Reverse: TATGTCATATTGTAAGTAAGTTGCAC

Source

We PCRed this part out of the pSG1154 gram-positive shuttle vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE153.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35	30,35	promoter, feature/promoter
-10	53,58	promoter, feature/promoter
Xylose Repressor Binding Region	68,91	operator, feature/operator

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K090502/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //regulation
• iGEM Parts Registry