BBa_K090505

BBa_K090505 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K090505

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Goodman

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:08:37

Bacillus subtilis consensus RBS

Details

• Types: DnaRegion
• Roles: ribosome_entry_site; RBS
• Sequences: BBa_K090505_sequence (Version 1)

Description

This is the consensus RBS for Bacillus subtilis.

Notes

The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AAAGGAGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.

Source

This part was synthesized directly.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
B. subtilis consensus RBS	1,8	ribosome_entry_site, feature/rbs

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K090505/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //direction/forward
• //rbs/prokaryote/constitutive/miscellaneous
• //regulation/constitutive
• //ribosome/prokaryote/bsubtilis
• iGEM Parts Registry