LOCUS       BBa_K091118             1985 bp    DNA     linear       18-May-2024
DEFINITION  codes for LasR and LasR regulated GFP
ACCESSION   BBa_K091118
VERSION     BBa_K091118.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..735
                     /label=lasR
     misc_feature    736..768
                     /label=LVA
     misc_feature    498..498
                     /label=G
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0079
     misc_feature    1..799
                     /label=BBa_S03156
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
     terminator      808..936
                     /label=BBa_B0015
     misc_feature    990..1007
                     /label=OP2
     misc_feature    1050..1069
                     /label=OP1
     promoter        1061..1066
                     /label=-35
     promoter        1084..1089
                     /label=-10
     promoter        945..1101
                     /label=BBa_R0079
     RBS             1110..1122
                     /label=RBS-3\Weak
     RBS             1116..1119
                     /label=RBS
     misc_feature    1110..1122
                     /label=BBa_B0032
     RBS             1110..1122
                     /label=BBa_B0032
     CDS             1129..1848
                     /label=GFP protein
     CDS             1129..1848
                     /label=BBa_E0040
     stem_loop       1868..1911
                     /label=stem_loop
     misc_feature    1857..1936
                     /label=BBa_B0010
     terminator      1857..1936
                     /label=BBa_B0010
     stem_loop       1952..1971
                     /label=T7 TE
     misc_feature    1978..1978
                     /label=stop
     polyA_site      1972..1985
                     /label=polya
     misc_feature    1945..1985
                     /label=BBa_B0012
     terminator      1945..1985
                     /label=BBa_B0012
     misc_feature    1110..1985
                     /label=BBa_E0240
ORIGIN
        1 aaagaggaga aatactagat ggccttggtt gacggttttc ttgagctgga acgctcaagt
       61 ggaaaattgg agtggagcgc catcctccag aagatggcga gcgaccttgg attctcgaag
      121 atcctgttcg gcctgttgcc taaggacagc caggactacg agaacgcctt catcgtcggc
      181 aactacccgg ccgcctggcg cgagcattac gaccgggctg gctacgcgcg ggtcgacccg
      241 acggtcagtc actgtaccca gagcgtactg ccgattttct gggaaccgtc catctaccag
      301 acgcgaaagc agcacgagtt cttcgaggaa gcctcggccg ccggcctggt gtatgggctg
      361 accatgccgc tgcatggtgc tcgcggcgaa ctcggcgcgc tgagcctcag cgtggaagcg
      421 gaaaaccggg ccgaggccaa ccgtttcata gagtcggtcc tgccgaccct gtggatgctc
      481 aaggactacg cactgcaaag cggtgccgga ctggccttcg aacatccggt cagcaaaccg
      541 gtggttctga ccagccggga gaaggaagtg ttgcagtggt gcgccatcgg caagaccagt
      601 tgggagatat cggttatctg caactgctcg gaagccaatg tgaacttcca tatgggaaat
      661 attcggcgga agttcggtgt gacctcccgc cgcgtagcgg ccattatggc cgttaatttg
      721 ggtcttatta ctctcgctgc aaacgacgaa aactacgctt tagtagctta ataacactga
      781 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttatatact agaggcccct cgctgagcgc
      961 gtcccggagc tgggggcaac ctagctgcca cctgcttttc tgctagctat tccagcgaaa
     1021 acatacagat ttccggcgaa atcaaggcta cctgccagtt ctggcaggtt tggccgcggg
     1081 ttctttttgg tacacgaaag ctactagagt cacacaggaa agtactagat gcgtaaagga
     1141 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1201 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     1261 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     1321 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     1381 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1441 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     1501 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     1561 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     1621 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     1681 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     1741 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     1801 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag
     1861 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1921 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1981 ttata
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