LOCUS       BBa_K091134             2048 bp    DNA     linear       10-May-2024
DEFINITION  Las Receiver with GFP reporter
ACCESSION   BBa_K091134
VERSION     BBa_K091134.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..798
                     /label=lasR
     misc_feature    799..831
                     /label=LVA
     misc_feature    561..561
                     /label=G
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0079
     misc_feature    64..862
                     /label=BBa_S03156
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     terminator      871..999
                     /label=BBa_B0015
     misc_feature    64..999
                     /label=BBa_S03954
     misc_feature    1..999
                     /label=BBa_S03966
     misc_feature    1053..1070
                     /label=OP2
     misc_feature    1113..1132
                     /label=OP1
     promoter        1124..1129
                     /label=-35
     promoter        1147..1152
                     /label=-10
     promoter        1008..1164
                     /label=BBa_R0079
     RBS             1173..1185
                     /label=RBS-3\Weak
     RBS             1179..1182
                     /label=RBS
     misc_feature    1173..1185
                     /label=BBa_B0032
     RBS             1173..1185
                     /label=BBa_B0032
     CDS             1192..1911
                     /label=GFP protein
     CDS             1192..1911
                     /label=BBa_E0040
     stem_loop       1931..1974
                     /label=stem_loop
     misc_feature    1920..1999
                     /label=BBa_B0010
     terminator      1920..1999
                     /label=BBa_B0010
     stem_loop       2015..2034
                     /label=T7 TE
     misc_feature    2041..2041
                     /label=stop
     polyA_site      2035..2048
                     /label=polya
     misc_feature    2008..2048
                     /label=BBa_B0012
     terminator      2008..2048
                     /label=BBa_B0012
     misc_feature    1008..2048
                     /label=BBa_S03956
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggccttg gttgacggtt ttcttgagct ggaacgctca
      121 agtggaaaat tggagtggag cgccatcctc cagaagatgg cgagcgacct tggattctcg
      181 aagatcctgt tcggcctgtt gcctaaggac agccaggact acgagaacgc cttcatcgtc
      241 ggcaactacc cggccgcctg gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac
      301 ccgacggtca gtcactgtac ccagagcgta ctgccgattt tctgggaacc gtccatctac
      361 cagacgcgaa agcagcacga gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg
      421 ctgaccatgc cgctgcatgg tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa
      481 gcggaaaacc gggccgaggc caaccgtttc atagagtcgg tcctgccgac cctgtggatg
      541 ctcaaggact acgcactgca aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa
      601 ccggtggttc tgaccagccg ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc
      661 agttgggaga tatcggttat ctgcaactgc tcggaagcca atgtgaactt ccatatggga
      721 aatattcggc ggaagttcgg tgtgacctcc cgccgcgtag cggccattat ggccgttaat
      781 ttgggtctta ttactctcgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagaggcc cctcgctgag
     1021 cgcgtcccgg agctgggggc aacctagctg ccacctgctt ttctgctagc tattccagcg
     1081 aaaacataca gatttccggc gaaatcaagg ctacctgcca gttctggcag gtttggccgc
     1141 gggttctttt tggtacacga aagctactag agtcacacag gaaagtacta gatgcgtaaa
     1201 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     1261 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     1321 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
     1381 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
     1441 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     1501 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     1561 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     1621 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     1681 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     1741 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     1801 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     1861 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc
     1921 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1981 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2041 cgtttata
//