LOCUS BBa_K091174 3852 bp DNA linear 01-Jun-2024 DEFINITION pBAD-RBS-TetR-TT-pBAD-RBS-LsrK-RBS-LsrR-TT ACCESSION BBa_K091174 VERSION BBa_K091174.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 CDS 160..776 /label=tetR misc_feature 777..810 /label=SsrA misc_feature 817..841 /label=Help:Barcodes CDS 157..816 /label=BBa_C0040 misc_feature 139..841 /label=BBa_S0101 misc_feature 1..841 /label=BBa_K091133 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 terminator 850..978 /label=BBa_B0015 misc_feature 1..978 /label=BBa_K091145 promoter 987..1116 /label=BBa_I13453 misc_feature 1129..1132 /label=conserved RBS 1125..1136 /label=BBa_B0034 CDS 1143..2735 /label=BBa_K091002 misc_feature 2748..2751 /label=conserved RBS 2744..2755 /label=BBa_B0034 CDS 2762..3715 /label=BBa_K091001 stem_loop 3735..3778 /label=stem_loop misc_feature 3724..3803 /label=BBa_B0010 terminator 3724..3803 /label=BBa_B0010 stem_loop 3819..3838 /label=T7 TE misc_feature 3845..3845 /label=stop polyA_site 3839..3852 /label=polya misc_feature 3812..3852 /label=BBa_B0012 terminator 3812..3852 /label=BBa_B0012 misc_feature 987..3852 /label=BBa_K091172 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa 181 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt 241 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag 301 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc 361 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt 421 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa 481 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag 541 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat 601 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca 661 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc 721 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct 781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagagacat tgattatttg cacggcgtca cactttgcta 1021 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact 1081 ctctactgtt tctccatacc gtttttttgg gctagctact agagaaagag gagaaatact 1141 agatggctcg actctttacc ctttcagaat caaagtacta cctgatggcg ctggatgcag 1201 gcaccggaag tattcgggct gtgatattcg acctggaagg caatcaaata gcagtgggac 1261 aggcggagtg gcggcatctg gcagtaccgg acgttcctgg ttctatggaa tttgatctca 1321 acaaaaactg gcaactggcg tgtgagtgta tgcgccaggc gctgcacaac gccggcatag 1381 ccccggagta tatcgctgcc gtttcggcat gttcgatgcg tgaaggcatt gttttatata 1441 ataatgaagg agccccgatc tgggcctgcg ccaatgtgga tgccagagcg gcacgcgaag 1501 ttagcgaact taaagaactg cacaacaata cctttgaaaa cgaagtttat cgcgcgaccg 1561 gacaaacact ggctttaagt gccatcccca gattactttg gctggcgcac catcgttccg 1621 atatttaccg tcaggcatca accatcacca tgatcagcga ctggctggcc tatatgctca 1681 gcggcgaact ggcggtggat ccctctaacg ctggcaccac gggacttctt gatctaacca 1741 cccgtgactg gaaacctgca ttgctggata tggctggcct acgtgccgat attctttctc 1801 ctgtcaaaga aaccggcaca ttgctgggcg tggtaagttc acaagcggcg gaactctgcg 1861 gtctgaaggc gggcactccg gtggtcgttg gaggaggcga cgtgcagctt ggttgccttg 1921 ggttaggcgt tgtgcgtccg gcacaaaccg cggttcttgg cggcacattc tggcagcaag 1981 ttgtaaattt agccgcgccg gtgacagacc cagaaatgaa cgtgcgcgtt aatcctcatg 2041 ttattcctgg catggtacaa gctgaatcta taagcttttt taccggactc accatgcgct 2101 ggttccgcga tgctttctgt gccgaagaaa aactgattgc cgaacgttta ggcatcgaca 2161 cctatacgct gctggaagag atggccagtc gggtgccgcc tgggtcgtgg ggcgtaatgc 2221 cgatcttctc cgacagaatg cgctttaaaa cctggtatca cgctgcgcct tcctttatta 2281 acttgtccat tgacccggat aaatgtaaca aagcgacatt gttccgtgcg ctggaagaaa 2341 atgcggcgat tgtatcagcg tgtaacttgc agcaaattgc tgatttctcg aatattcatc 2401 cttcatcgtt agtctttgca ggcggaggtt caaaagggaa attatggagt caaattctcg 2461 ctgatgtctc gggattaccc gtcaatattc cggtggtcaa agaagccact gcattaggat 2521 gtgccattgc agctggcgtc ggtgccggaa ttttttcatc aatggcagaa accggagaac 2581 gcctggttcg ctgggaacgg acgcacacac cagacccgga aaagcatgaa ctttatcagg 2641 attcacgcga taagtggcag gcagtttatc aggatcagct ggggctggtt gatcatggac 2701 tgacgacgtc gttatggaaa gcgcctgggt tatagtacta gagaaagagg agaaatacta 2761 gatgacaatc aacgattcgg caatttcaga acagggaatg tgtgaagaag aacaggtcgc 2821 gcggatcgcg tggttttact atcacgacgg gctgacccag agcgagatca gcgatcgtct 2881 cggcctgaca cgtttgaaag tgtcgcgatt gctggagaaa gggcatcagt ccggcattat 2941 tcgcgtacag attaattctc gctttgaagg ctgtctggaa tatgaaactc aattacgtcg 3001 tcagttttcg ctgcaacatg tccgggtgat ccctgggctt gcggatgctg atgtcggtgg 3061 gcgactgggg ataggcgcgg cgcatatgtt gatgagttta cttcaaccac aacagatgct 3121 ggcgattggt tttggcgagg caaccatgaa tacgctgcaa cgcttaagtg gttttatttc 3181 gtcacagcaa attcgcctgg tcacgctctc cggtggcgtc ggttcttata tgacgggaat 3241 cgggcagctt aacgcggcgt gcagtgtgaa tattattccg gctccgttgc gggcatcctc 3301 cgctgacatt gcccgtacgc taaaaaatga aaattgcgtc aaagatgttc tgttagccgc 3361 gcaagcagcg gatgtggcga ttgtcggcat tggtgctgtg agtcaacagg acgatgcgac 3421 aatcattcgc tccggttata tcagccaggg cgaacagtta atgattggcc gaaaaggggc 3481 ggttggcgac attttaggct acttttttga tgcaaaaggt gacgttgtca cgaatatcaa 3541 aatacataac gaactgattg gcttaccttt aagcgcgctg aagaccatac ccgtccgggt 3601 tggcgtggca gggggagaaa ataaagccga agcaattgcc gctgcaatga aaggcggtta 3661 tatcaacgca ctggttaccg atcaggacac agcagcggcg attttacgta gttaatacta 3721 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 3781 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 3841 tctgcgttta ta //