LOCUS       BBa_K091194             2023 bp    DNA     linear       01-Jun-2024
DEFINITION  Promoter testing construct
ACCESSION   BBa_K091194
VERSION     BBa_K091194.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux Box
     promoter        20..25
                     /label=Lux -35
     misc_feature    26..43
                     /label=cI OR2
     promoter        44..49
                     /label=Lux -10
     promoter        1..57
                     /label=BBa_K091107
     RBS             66..78
                     /label=RBS-3\Weak
     RBS             72..75
                     /label=RBS
     misc_feature    66..78
                     /label=BBa_B0032
     RBS             66..78
                     /label=BBa_B0032
     CDS             85..804
                     /label=GFP protein
     CDS             85..804
                     /label=BBa_E0040
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     misc_feature    1..941
                     /label=BBa_K091165
     promoter        950..1079
                     /label=BBa_I13453
     misc_feature    1092..1095
                     /label=conserved
     RBS             1088..1099
                     /label=BBa_B0034
     misc_feature    1106..1107
                     /label=prefix
     misc_feature    1279..1279
                     /label=T
     misc_feature    1597..1597
                     /label=A
     CDS             1106..1855
                     /label=luxR
     misc_feature    1106..1861
                     /label=BBa_C0062
     misc_feature    1862..1886
                     /label=Help:Barcodes
     CDS             1106..1861
                     /label=BBa_C0062
     misc_feature    1088..1886
                     /label=BBa_J37033
     misc_feature    950..1886
                     /label=BBa_K091166
     stem_loop       1906..1949
                     /label=stem_loop
     misc_feature    1895..1974
                     /label=BBa_B0010
     terminator      1895..1974
                     /label=BBa_B0010
     stem_loop       1990..2009
                     /label=T7 TE
     misc_feature    2016..2016
                     /label=stop
     polyA_site      2010..2023
                     /label=polya
     misc_feature    1983..2023
                     /label=BBa_B0012
     terminator      1983..2023
                     /label=BBa_B0012
     terminator      1895..2023
                     /label=BBa_B0015
     misc_feature    950..2023
                     /label=BBa_K091179
ORIGIN
        1 acctgtagga tcgtacaggt ttacgtaaca ccgtgcgtgt tgatatagtc gaataaatac
       61 tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac tggagttgtc
      121 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      181 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      241 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
      301 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
      361 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
      421 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
      481 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
      541 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
      601 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
      661 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
      721 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
      781 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga cattgattat
      961 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
     1021 acctgacgct ttttatcgca actctctact gtttctccat accgtttttt tgggctagct
     1081 actagagaaa gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat
     1141 aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat
     1201 gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt
     1261 taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga
     1321 cgctaattta ataaaatatg atcctatagt agattattct aactccaatc attcaccaat
     1381 taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga
     1441 agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg
     1501 cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt
     1561 acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat
     1621 aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc
     1681 gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg
     1741 tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca
     1801 aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata
     1861 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
     1921 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1981 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//