LOCUS       BBa_K091195             1983 bp    DNA     linear       01-Jun-2024
DEFINITION  Promoter testing construct
ACCESSION   BBa_K091195
VERSION     BBa_K091195.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux Box
     promoter        20..25
                     /label=Lux -35
     misc_feature    26..43
                     /label=cI OR2
     promoter        44..49
                     /label=Lux -10
     promoter        1..57
                     /label=BBa_K091107
     RBS             66..78
                     /label=RBS-3\Weak
     RBS             72..75
                     /label=RBS
     misc_feature    66..78
                     /label=BBa_B0032
     RBS             66..78
                     /label=BBa_B0032
     CDS             85..804
                     /label=GFP protein
     CDS             85..804
                     /label=BBa_E0040
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     misc_feature    1..941
                     /label=BBa_K091165
     promoter        950..1079
                     /label=BBa_I13453
     misc_feature    1092..1095
                     /label=conserved
     RBS             1088..1099
                     /label=BBa_B0034
     CDS             1109..1816
                     /label=cI lambda
     misc_feature    1817..1849
                     /label=LVA
     misc_feature    1856..1880
                     /label=Help:Barcodes
     CDS             1106..1855
                     /label=BBa_C0051
     stem_loop       1896..1915
                     /label=T7 TE
     misc_feature    1922..1922
                     /label=stop
     polyA_site      1916..1929
                     /label=polya
     misc_feature    1889..1929
                     /label=BBa_B0012
     terminator      1889..1929
                     /label=BBa_B0012
     stem_loop       1950..1972
                     /label=stem_loop
     misc_feature    1938..1983
                     /label=BBa_B0011
     terminator      1938..1983
                     /label=BBa_B0011
     misc_feature    1088..1983
                     /label=BBa_P0455
     misc_feature    950..1983
                     /label=BBa_K091171
ORIGIN
        1 acctgtagga tcgtacaggt ttacgtaaca ccgtgcgtgt tgatatagtc gaataaatac
       61 tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac tggagttgtc
      121 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      181 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      241 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
      301 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
      361 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
      421 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
      481 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
      541 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
      601 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
      661 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
      721 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
      781 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga cattgattat
      961 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
     1021 acctgacgct ttttatcgca actctctact gtttctccat accgtttttt tgggctagct
     1081 actagagaaa gaggagaaat actagatgag cacaaaaaag aaaccattaa cacaagagca
     1141 gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt
     1201 atcccaggaa tctgtcgcag acaagatggg gatggggcag tcaggcgttg gtgctttatt
     1261 taatggcatc aatgcattaa atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt
     1321 tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc tacgagatgt atgaagcggt
     1381 tagtatgcag ccgtcactta gaagtgagta tgagtaccct gttttttctc atgttcaggc
     1441 agggatgttc tcacctgagc ttagaacctt taccaaaggt gatgcggaga gatgggtaag
     1501 cacaaccaaa aaagccagtg attctgcatt ctggcttgag gttgaaggta attccatgac
     1561 cgcaccaaca ggctccaagc caagctttcc tgacggaatg ttaattctcg ttgaccctga
     1621 gcaggctgtt gagccaggtg atttctgcat agccagactt gggggtgatg agtttacctt
     1681 caagaaactg atcagggata gcggtcaggt gtttttacaa ccactaaacc cacagtaccc
     1741 aatgatccca tgcaatgaga gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc
     1801 tgaagagacg tttggcgctg caaacgacga aaactacgct ttagtagctt aataacgctg
     1861 atagtgctag tgtagatcgc tactagagtc acactggctc accttcgggt gggcctttct
     1921 gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta
     1981 ttt
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