LOCUS       BBa_K091213             2248 bp    DNA     linear       10-May-2024
DEFINITION  LacI X86 and pLac promoter upstream of GFP
ACCESSION   BBa_K091213
VERSION     BBa_K091213.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    261..263
                     /label=serine->leucine
     CDS             81..1163
                     /label=BBa_K091124
     stem_loop       1183..1226
                     /label=stem_loop
     misc_feature    1172..1251
                     /label=BBa_B0010
     terminator      1172..1251
                     /label=BBa_B0010
     stem_loop       1267..1286
                     /label=T7 TE
     misc_feature    1293..1293
                     /label=stop
     polyA_site      1287..1300
                     /label=polya
     misc_feature    1260..1300
                     /label=BBa_B0012
     terminator      1260..1300
                     /label=BBa_B0012
     promoter        1325..1330
                     /label=-35
     promoter        1352..1357
                     /label=-10
     promoter        1309..1364
                     /label=BBa_K091110
     RBS             1373..1385
                     /label=RBS-3\Weak
     RBS             1379..1382
                     /label=RBS
     misc_feature    1373..1385
                     /label=BBa_B0032
     RBS             1373..1385
                     /label=BBa_B0032
     CDS             1392..2111
                     /label=GFP protein
     CDS             1392..2111
                     /label=BBa_E0040
     stem_loop       2131..2174
                     /label=stem_loop
     misc_feature    2120..2199
                     /label=BBa_B0010
     terminator      2120..2199
                     /label=BBa_B0010
     stem_loop       2215..2234
                     /label=T7 TE
     misc_feature    2241..2241
                     /label=stop
     polyA_site      2235..2248
                     /label=polya
     misc_feature    2208..2248
                     /label=BBa_B0012
     terminator      2208..2248
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag ctgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgatactaga gccaggcatc aaataaaacg aaaggctcag
     1201 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1261 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagcg ttgacaccat
     1321 cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggtactag agtcacacag
     1381 gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     1441 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     1501 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     1561 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
     1621 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     1681 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     1741 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     1801 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     1861 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     1921 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     1981 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     2041 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     2101 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     2161 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     2221 ccttcgggtg ggcctttctg cgtttata
//