LOCUS       BBa_K092900             2102 bp    DNA     linear       17-May-2024
DEFINITION  RhlR controlled by a constitutive promoter, and LacI modified with 
            a Rhl R promoter
ACCESSION   BBa_K092900
VERSION     BBa_K092900.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     CDS             65..793
                     /label=BBa_C0171
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     misc_feature    939..958
                     /label=RhlR
     promoter        957..962
                     /label=-35
     promoter        980..985
                     /label=-10
     misc_feature    991..991
                     /label=start
     promoter        939..991
                     /label=BBa_R0071
     misc_feature    1000..1011
                     /label=B0034
     CDS             1020..2102
                     /label=LacIm
     misc_feature    1000..2102
                     /label=BBa_K092800
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatgagg aatgacggag gctttttgct gtggtgggac ggtttgcgta gcgagatgca
      121 gccgatccac gacagccagg gcgtgttcgc cgtcctggaa aaggaagtgc ggcgcctggg
      181 cttcgattac tacgcctatg gcgtgcgcca cacgattccc ttcacccggc cgaagaccga
      241 ggtccatggc acctatccca aggcctggct ggagcgatac cagatgcaga actacggggc
      301 cgtggatccg gcgatcctca acggcctgcg ctcctcggaa atggtggtct ggagcgacag
      361 cctgttcgac cagagccgga tgctctggaa cgaggctcgc gattggggcc tctgtgtcgg
      421 cgcgaccttg ccgatccgcg cgccgaacaa tttgctcagc gtgctttccg tggcgcgcga
      481 ccagcagaac atctccagct tcgagcgcga ggaaatccgc ctgcggctgc gttgcatgat
      541 cgagttgctg acccagaagc tgaccgacct ggagcatccg atgctgatgt ccaacccggt
      601 ctgcctgagc catcgcgaac gcgagatcct gcaatggacc gccgacggca agagttccgg
      661 ggaaatcgcc atcatcctga gcatctccga gagcacggtg aacttccacc acaagaacat
      721 ccagaagaag ttcgacgcgc cgaacaagac gctggctgcc gcctacgccg cggcgctggg
      781 tctcatctaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagtc ctgtgaaatc tggcagttac
      961 cgttagcttt cgaattggct aaaaagtgtt ctactagaga aagaggagaa atactagagt
     1021 cattggccag actctaagcg gctcacttgg cgcgctaatt gcatcaggct gtccgccagg
     1081 gcacgcgggc tcgccgtctg ggtgttcggg gctaacgtcg tcttgcgctt aactaagctc
     1141 accggcagta attggttacc tttaacggct tgaccttggc tcagctgtaa cagacgatca
     1201 acagacgtct ggcctaataa acggaagtct tgcttaatcg tcgtgcttgg tggaatgtag
     1261 cagctagaat cctccgtgtc atcatagcca accacgctaa tgtcggcgcc cacacgtaag
     1321 ccgctttccg tgatcgcacg catcgcacct aaggccattt ggtcattcgc cactaacatg
     1381 gcggtcggca caataccttc gtttaacatc tgcatcgtct gctggaagcc gctcatcgcg
     1441 ctccaatcac cctcgcgctc ggcaattggt tggatctggt tacgggtcag gtacttgtgc
     1501 caacccgcta agcgtaagcg ggcgctcacg ctgctcagcg gaccggccag taaggcaatc
     1561 tgttgatggc ccagcgccac taagtgttca acacctaagc gggtgccatc ctcgtggcta
     1621 aagatgatgc tattaatcgg ggtttgatcg ctcacgtcca ggaacagggc tggcacgttg
     1681 gtacacgccg cctcaaccgc gatcgcgtct tgatcgtcta atgggtaatt gataattaag
     1741 ccgctcacac gctgggccag caggttatga acggcggcct tgcacgcctc cacaccagag
     1801 cgctccacca tgctaacaac aacagacgcg cctaactggt ccgcacggct cttgatggcg
     1861 gccacgatct ggcttggggc atgtaacgct aagctgctcg tcgccacacc gattaacagg
     1921 ctttgcttac cggctaactg ctgcgcaaca cgattcggga tatagtttaa ttcggccatg
     1981 gcggcctcaa ccttctcgcg ggtcttggcg ctcacatgag acgcttgatt aacaacacgg
     2041 ctcaccgttt ggtagctcac gcccgcgtat tccgccacgt catacagggt caccggcttc
     2101 at
//