P3

BBa_K100002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K100002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Garrett Tobin
Date created: 2008-08-17 11:00:00
Date modified: 2015-05-08 01:08:41

Edited Xylose Regulated Bi-Directional Operator 2



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K100002_sequence (Version 1)

Description

This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has a scrambled CRP binding site and and altered RNA ploymerase binding site that was designed to more resemble the consensus sequence at an intermediate level.

Notes

Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control. The RNA polymerase binding site was altered to make binding stronger, attempting to allow activation when XylR-xylose alone is bound (the absence of CRP-cAMP).

Source

Taken from E. Coli genome bases 3728830-3729092, base pairs changed at RNA polymerase bining sites.

Sequence Annotation Location Component / Role(s)
RNA poly. Binding Site
RNA poly. Binding Site
Scrambled CRP Binding Site
RNA poly. Binding Site
RNA poly. Binding Site
45,50
21,26
88,104
243,248
265,270
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
sequence_feature feature/misc
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K100002/1