LOCUS       BBa_K101006              849 bp    DNA     linear       11-May-2024
DEFINITION  Construct with TetR/p22mnt promoter, RBS, RFP, Terminator
ACCESSION   BBa_K101006
VERSION     BBa_K101006.1  
FEATURES             Location/Qualifiers
     promoter        1..61
                     /label=BBa_K101000
     RBS             70..82
                     /label=RBS-3\Weak
     RBS             76..79
                     /label=RBS
     misc_feature    70..82
                     /label=BBa_B0032
     RBS             70..82
                     /label=BBa_B0032
     CDS             89..799
                     /label=mCherry
     misc_feature    440..440
                     /label=C->T (removing PstI site)
     misc_feature    89..802
                     /label=BBa_J06504
     misc_feature    811..849
                     /label=B1006
     stem_loop       820..841
                     /label=modified thr terminator
     polyA_site      842..849
                     /label=PolyA
     polyA_site      811..819
                     /label=PolyA
     terminator      811..849
                     /label=BBa_B1006
ORIGIN
        1 tccctatcag tgatagagat tgacaaggtc cacggtgacc tagatctccg atactgagca
       61 ctactagagt cacacaggaa agtactagat ggtgagcaag ggcgaggagg ataacatggc
      121 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      181 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      241 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      301 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      361 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      421 cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      481 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      541 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
      601 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      661 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
      721 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
      781 ggacgagctg tacaagtaat aatactagag aaaaaaaaac cccgcccctg acagggcggg
      841 gtttttttt
//