BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898429
1
modified thr terminator
range1898429
1
10
31
annotation1898431
1
PolyA
range1898431
1
1
9
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898428
1
B1006
range1898428
1
1
39
BBa_K101006
1
BBa_K101006
Construct with TetR/p22mnt promoter, RBS, RFP, Terminator
2008-07-27T11:00:00Z
2015-05-08T01:08:41Z
Construct is composed of: TetR promoter (R0040), p22mnt promoter (R0073), RBS (B0032), RFP (J06504), and Terminator (B1006).
TetR is a promoter. p22mnt is a promoter. RBS is the ribosomal binding site. RFP is the red fluorescent protein, which is a reporter gene. The terminator will terminate transcription.
false
false
_205_
0
3370
9
It's complicated
false
Majority of design considerations were dealt with during the design of the dually repressed promoter (K101000). A medium strength RBS was used to increase the affinity of this part for ribosomal binding. Additionally, this decision was made to use only a single terminator.
false
Ellen Martin
component1968852
1
BBa_B1006
component1968843
1
BBa_B0032
component1968847
1
BBa_J06504
component1968841
1
BBa_K101000
annotation1968852
1
BBa_B1006
range1968852
1
811
849
annotation1968843
1
BBa_B0032
range1968843
1
70
82
annotation1968847
1
BBa_J06504
range1968847
1
89
802
annotation1968841
1
BBa_K101000
range1968841
1
1
61
BBa_K101000
1
BBa_K101000
Dual-Repressed Promoter for p22 mnt and TetR
2008-07-06T11:00:00Z
2015-05-08T01:08:41Z
This part was designed by combining sequences from parts C0040 (TetR promoter) and C0072 (p22 mnt promoter).
This part is a promoter that is repressed by both p22 mnt and TetR. It can be used to control the response of any gene that follows this promoter. It will be repressed by the presence of either p22 mnt or TetR.
false
false
_205_
0
3363
9
Not in stock
false
TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
false
Bennett Swiniarski
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_J06504
1
mCherry
monomeric RFP optimized for bacteria
2005-07-17T11:00:00Z
2016-01-25T01:05:28Z
mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
Released HQ 2013
mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
false
false
_20_
4206
340
20
In stock
false
<p>
Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR.
</p>
<p>
Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
</p>
true
ytwang
annotation1585833
1
C->T (removing PstI site)
range1585833
1
352
352
annotation1585829
1
mCherry
range1585829
1
1
711
BBa_J06504_sequence
1
atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
BBa_K101000_sequence
1
tccctatcagtgatagagattgacaaggtccacggtgacctagatctccgatactgagcac
BBa_B0032_sequence
1
tcacacaggaaag
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_K101006_sequence
1
tccctatcagtgatagagattgacaaggtccacggtgacctagatctccgatactgagcactactagagtcacacaggaaagtactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z