LOCUS       BBa_K101013              854 bp    DNA     linear       09-May-2024
DEFINITION  Construct with TetR/p22cII promoter, RBS, RFP, Terminator
ACCESSION   BBa_K101013
VERSION     BBa_K101013.1  
FEATURES             Location/Qualifiers
     promoter        1..66
                     /label=BBa_K101002
     RBS             75..87
                     /label=RBS-3\Weak
     RBS             81..84
                     /label=RBS
     misc_feature    75..87
                     /label=BBa_B0032
     RBS             75..87
                     /label=BBa_B0032
     CDS             94..804
                     /label=mCherry
     misc_feature    445..445
                     /label=C->T (removing PstI site)
     misc_feature    94..807
                     /label=BBa_J06504
     misc_feature    816..854
                     /label=B1006
     stem_loop       825..846
                     /label=modified thr terminator
     polyA_site      847..854
                     /label=PolyA
     polyA_site      816..824
                     /label=PolyA
     terminator      816..854
                     /label=BBa_B1006
ORIGIN
        1 tccctatcag tgatagagat tgactaaaga ttcctttagt agataattta agtgttcttt
       61 aatttctact agagtcacac aggaaagtac tagatggtga gcaagggcga ggaggataac
      121 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      181 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      241 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      301 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      361 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      421 gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa ggtgaagctg
      481 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      541 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      601 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      661 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      721 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      781 ggcatggacg agctgtacaa gtaataatac tagagaaaaa aaaaccccgc ccctgacagg
      841 gcggggtttt tttt
//