LOCUS       BBa_K101014             1033 bp    DNA     linear       18-May-2024
DEFINITION  AraC promoter, RBS, GFP and terminator
ACCESSION   BBa_K101014
VERSION     BBa_K101014.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     RBS             158..170
                     /label=RBS-3\Weak
     RBS             164..167
                     /label=RBS
     misc_feature    158..170
                     /label=BBa_B0032
     RBS             158..170
                     /label=BBa_B0032
     CDS             177..896
                     /label=GFP protein
     CDS             177..896
                     /label=BBa_E0040
     stem_loop       916..959
                     /label=stem_loop
     misc_feature    905..984
                     /label=BBa_B0010
     terminator      905..984
                     /label=BBa_B0010
     stem_loop       1000..1019
                     /label=T7 TE
     misc_feature    1026..1026
                     /label=stop
     polyA_site      1020..1033
                     /label=polya
     misc_feature    993..1033
                     /label=BBa_B0012
     terminator      993..1033
                     /label=BBa_B0012
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagagtca cacaggaaag tactagatgc
      181 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      241 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      301 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      361 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
      421 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
      481 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
      541 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
      601 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
      661 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
      721 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
      781 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
      841 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact
      901 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      961 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1021 ttctgcgttt ata
//