LOCUS       BBa_K1014009            1059 bp    DNA     linear       14-Jun-2024
DEFINITION  tetR Protein Generator(M)
ACCESSION   BBa_K1014009
VERSION     BBa_K1014009.1  
FEATURES             Location/Qualifiers
     promoter        1..210
                     /label=BBa_K1014002
     RBS             219..231
                     /label=RBS-3\Weak
     RBS             225..228
                     /label=RBS
     misc_feature    219..231
                     /label=BBa_B0032
     RBS             219..231
                     /label=BBa_B0032
     CDS             241..857
                     /label=tetR
     misc_feature    858..891
                     /label=SsrA
     misc_feature    898..922
                     /label=Help:Barcodes
     CDS             238..922
                     /label=BBa_C0040
     stem_loop       942..985
                     /label=stem_loop
     misc_feature    931..1010
                     /label=BBa_B0010
     terminator      931..1010
                     /label=BBa_B0010
     stem_loop       1026..1045
                     /label=T7 TE
     misc_feature    1052..1052
                     /label=stop
     polyA_site      1046..1059
                     /label=polya
     misc_feature    1019..1059
                     /label=BBa_B0012
     terminator      1019..1059
                     /label=BBa_B0012
ORIGIN
        1 gccggattat gtccgctgag tgggtcacgg tcccggatca gttcccttgc gaagctgacc
       61 gatgtttttg atgccaaaag ctgttgtggc aaaaaacggt ttgcgcaaag ttttgtatta
      121 caaagaattt cacattttaa atatatcttt ataaatcaat cagttatttc tatttttaag
      181 ctggcatggt tatcgctata gggcttgtac tactagagtc acacaggaaa gtactagatg
      241 tccagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga
      301 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg
      361 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat
      421 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat
      481 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta
      541 ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc
      601 caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact
      661 ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct
      721 actactgata gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt
      781 gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt
      841 aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac
      901 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1021 acactggctc accttcgggt gggcctttct gcgtttata
//