BBa_K137010
1
fimE IRL
fimE IRL
2008-06-19T11:00:00Z
2015-05-08T01:10:08Z
pFIP plasmid
fimE inverted repeat left recombination site
false
false
_187_
0
3112
9
It's complicated
false
none
false
Allen Lin
annotation1963844
1
IRL out
range1963844
1
19
35
annotation1963843
1
IRL in
range1963843
1
1
17
BBa_K1015014
1
BBa_K1015014
IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)
2013-09-14T11:00:00Z
2015-05-08T01:08:42Z
BBa_K1015000 BBa_K137008 BBa_K137047 BBa_K137010 B0034 BBa_K1015001 BBa_K1015002 BBa_K1015003
With acteriophage λ recombination sysytem, this device insert into Escherichia coli genome.
This device produce FLP and AadA(streptomycin resistant gene) .
But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop.
false
false
_1321_
0
15618
9
It's complicated
true
genome insertion parts
false
Kento Tominaga
component2374424
1
BBa_K137047
component2374422
1
BBa_K137008
component2374427
1
BBa_K137010
component2374431
1
BBa_K1015001
component2374419
1
BBa_K1015000
component2374435
1
BBa_K1015002
component2374437
1
BBa_K1015003
component2374429
1
BBa_B0034
annotation2374429
1
BBa_B0034
range2374429
1
389
400
annotation2374437
1
BBa_K1015003
range2374437
1
2726
2761
annotation2374422
1
BBa_K137008
range2374422
1
45
79
annotation2374419
1
BBa_K1015000
range2374419
1
1
36
annotation2374424
1
BBa_K137047
range2374424
1
88
337
annotation2374431
1
BBa_K1015001
range2374431
1
407
1678
annotation2374435
1
BBa_K1015002
range2374435
1
1687
2717
annotation2374427
1
BBa_K137010
range2374427
1
346
380
BBa_K1015002
1
BBa_K1015002
streptmycin resisitance gene (can PCR ampicillin resistance gene primer)
2013-09-14T11:00:00Z
2016-01-28T01:50:11Z
aminoglycoside resistance gene (aadA) come from R100.1 plasmid.
streptmycin resisitance gene(aadA)
This aadA gene are inserted into blaZ gene come from pI258 plasmid. So this aadA gene can PCR with primers for blaZ.
false
false
_1321_
4206
15618
9
Not in stock
false
antibiotic resistance
false
Kento Tominaga
annotation2342880
1
aadA(streptomycin resistance gene)
range2342880
1
119
911
annotation2342888
1
blaZ fragment
range2342888
1
911
1030
annotation2342881
1
blaZ fragment(promoter contain)
range2342881
1
1
118
BBa_K1015003
1
BBa_K1015003
cynR homology sequence(for RED recombination system)
2013-09-14T11:00:00Z
2015-05-08T01:08:42Z
Escherichia coli MG1655 cynR gene
This sequence is 36bp homologous sequence with Escherichia coli cynR gene. Bacteriophage λ recombination system(RED system) can cause homologous recombination between this sequence and cynR gene. Because of the recombination, Biobrick part can insert into E.coli genome.
false
false
_1321_
0
15618
9
Not in stock
false
Genome insertion
false
Kento Tominaga
annotation2342941
1
cynR homology sequence
range2342941
1
1
36
BBa_K1015001
1
BBa_K1015001
Flippase(FLP)
2013-09-14T11:00:00Z
2015-05-08T01:08:42Z
2??m plasmid of Saccharomyces cerevisiae.
site specific recombinase that recognize FRT site.
FLP catalyze site specific recombination between two FRT site.
false
false
_1321_
0
15618
9
Not in stock
false
site specific recombination between two FRT site.
false
Kento Tominaga
annotation2342868
1
FLP
range2342868
1
1
1272
BBa_K137008
1
IRR
fimE IRR
2008-06-19T11:00:00Z
2015-05-08T01:10:08Z
pFIP plasmid
fimE inverted repeat right recombination site
false
false
_187_
0
3112
9
It's complicated
false
none
false
Allen Lin
annotation1963842
1
IRR in
range1963842
1
19
35
annotation1963841
1
IRR out
range1963841
1
1
17
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K137047
1
BBa_K137047
250 bp inverted tetR promoter
2008-07-20T11:00:00Z
2015-05-08T01:10:09Z
Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
Inverted tetR promoter with noncoding, spacer DNA downstream of it to make the total length 250 bp. The promoter faces the 5' direction.
false
false
_187_
0
3112
9
It's complicated
false
This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
false
Allen Lin
annotation1968020
1
tetR promoter
range1968020
1
1
54
BBa_K1015000
1
BBa_K1015000
lacA-homology sequence(for RED recombination system)
2013-09-14T11:00:00Z
2015-05-08T01:08:42Z
CGGAAGTCATGTGGTTATTAATCCAGGCGTCACCAT
This sequence is 36bp homologous sequence with Escherichia coli lacA gene.
Bacteriophage λ recombination system(RED system) can cause homologous recombination between this sequence and lacA gene.
Because of the recombination, Biobrick part can insert into E.coli genome.
false
false
_1321_
0
15618
9
Not in stock
false
Genome insertion
false
Kento Tominaga
annotation2343703
1
lacA homology sequence
range2343703
1
1
36
BBa_K137010_sequence
1
tctatgagtcaaaatggccccaattgtcttgtatt
BBa_K1015003_sequence
1
tcacggtagccgctgacacaggtctgcggctggaat
BBa_K137008_sequence
1
aagatgaaacatttggggccaaactgtccatatta
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1015001_sequence
1
atgccacaatttggtatattatgtaaaacaccacctaaggtgcttgttcgtcagtttgtggaaaggtttgaaagaccttcaggtgagaaaatagcattatgtgctgctgaactaacctatttatgttggatgattacacataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaataaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattgattcctgcttgggaatttacaattattccttactatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacttcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgaggaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaataaacaggaataccaattattaaaagataacttagtcagatcgtacaataaagctttgaagaaaaatgcgccttattcaatctttgctataaaaaatggcccaaaatctcacattggaagacatttgatgacctcatttctttcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcactagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatataa
BBa_K1015014_sequence
1
cggaagtcatgtggttattaatccaggcgtcaccattactagagaagatgaaacatttggggccaaactgtccatattatactagaggtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagtttttactagagtctatgagtcaaaatggccccaattgtcttgtatttactagagaaagaggagaaatactagatgccacaatttggtatattatgtaaaacaccacctaaggtgcttgttcgtcagtttgtggaaaggtttgaaagaccttcaggtgagaaaatagcattatgtgctgctgaactaacctatttatgttggatgattacacataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaataaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattgattcctgcttgggaatttacaattattccttactatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacttcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgaggaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaataaacaggaataccaattattaaaagataacttagtcagatcgtacaataaagctttgaagaaaaatgcgccttattcaatctttgctataaaaaatggcccaaaatctcacattggaagacatttgatgacctcatttctttcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcactagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatataatactagagtaacttagccatttcaacaccttctttcaaatatttataataaactattgacaccgatattacaattgtaatattattgatttataaaaattacaactgtaatatcggagggtttatttatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataaatattctaaatgcataataaatactgataacatcttatattttgtattatattttgtattatcgttgacatgtataattttgatatcaaaaactgattttccctctattattttcgagattactagagtcacggtagccgctgacacaggtctgcggctggaat
BBa_K137047_sequence
1
gtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagtttt
BBa_K1015000_sequence
1
cggaagtcatgtggttattaatccaggcgtcaccat
BBa_K1015002_sequence
1
taacttagccatttcaacaccttctttcaaatatttataataaactattgacaccgatattacaattgtaatattattgatttataaaaattacaactgtaatatcggagggtttatttatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataaatattctaaatgcataataaatactgataacatcttatattttgtattatattttgtattatcgttgacatgtataattttgatatcaaaaactgattttccctctattattttcgagat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z