LOCUS       BBa_K1017781            1482 bp    DNA     linear       19-May-2024
DEFINITION  LacI+J61048+Plac
ACCESSION   BBa_K1017781
VERSION     BBa_K1017781.1  
FEATURES             Location/Qualifiers
     misc_feature    1090..1128
                     /label=LVA
     CDS             1..1128
                     /label=lacI-LVA
     misc_feature    1..1128
                     /label=BBa_C0012
     misc_feature    1129..1153
                     /label=Help:Barcodes
     CDS             1..1128
                     /label=BBa_C0012
     terminator      1162..1274
                     /label=BBa_J61048
     CDS             1283..1370
                     /label=end of LacI coding region (inactive)
     misc_feature    1283..1482
                     /label=BBa_R0010
     misc_feature    1371..1408
                     /label=CAP binding site
     promoter        1419..1424
                     /label=-35
     promoter        1443..1448
                     /label=-10
     misc_feature    1448..1482
                     /label=LacI binding site
     misc_feature    1455..1455
                     /label=start
     promoter        1283..1482
                     /label=BBa_R0010
ORIGIN
        1 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
       61 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
      121 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
      181 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
      241 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
      301 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
      361 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
      421 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
      481 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
      541 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
      601 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
      661 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
      721 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
      781 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
      841 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
      901 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
      961 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     1021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1081 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     1141 tagtgtagat ctctactaga gccggcttat cggtcagttt cacctgattt acgtaaaaac
     1201 ccgcttcggc gggtttttgc ttttggaggg gcagaaagat gaatgactgt ccacgacgct
     1261 atacccaaaa gaaatactag agcaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1321 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     1381 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     1441 cgtatgttgt gtggaattgt gagcggataa caatttcaca ca
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